概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to ARIH2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Drosophila melanogaster
  • 免疫原

    Vector coding for a partial recombinant fusion protein, (His tagged), corresponding to amino acids 11-41 of Fruit fly (Drosophila melanogaster) ARIH2. Target sequence used to make the antibody:

    SDNDGEYDDDYDYYNTGEDCDVERLDPKRA

    .

  • 常规说明

     

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 纯度
    Whole antiserum
  • Primary antibody说明
    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 59 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能
    E3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. May play a role in myelopoiesis.
  • 组织特异性
    Widely expressed with higher expression in granulocytes.
  • 通路
    Protein modification; protein ubiquitination.
  • 序列相似性
    Belongs to the RBR family. Ariadne subfamily.
    Contains 1 IBR-type zinc finger.
    Contains 2 RING-type zinc fingers.
  • 结构域
    RING-type 1 and RING-type 2 are required for the inhibitory function in myelopoiesis.
  • 翻译后修饰
    Ubiquitinated. Ubiquitination promotes proteasomal degradation.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus. Cytoplasm.
  • Information by UniProt
  • 别名
    • HT005 antibody
    • all trans retinoic acid inducible RING finger antibody
    • Ari 2 antibody
    • Ari-2 antibody
    • ARI2 antibody
    • ARI2_HUMAN antibody
    • Ariadne 2 antibody
    • Ariadne 2 protein homolog antibody
    • ariadne homolog 2 (Drosophila) antibody
    • ariadne homolog 2 antibody
    • Ariadne RBR E3 ubiquitin protein ligase 2 antibody
    • ARIH 2 antibody
    • Arih2 antibody
    • E3 ubiquitin protein ligase ARIH2 antibody
    • E3 ubiquitin-protein ligase ARIH2 antibody
    • protein ariadne 2 homolog antibody
    • Protein ariadne-2 homolog antibody
    • Triad 1 antibody
    • TRIAD1 antibody
    • Triad1 protein antibody
    • UIP48 antibody
    see all

Anti-ARIH2 antibody 图像

  • All lanes : Anti-ARIH2 antibody (ab52678) at 1/1000 dilution

    Lane 1 : Total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen
    Lane 2 : Total protein extract from E coli with 50ng to 500ng of the antigen (recombinant fusion protein containing a His tag).

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, HRP conjugated, at 1/5000 dilution

    Predicted band size : 59 kDa

实验方案

Anti-ARIH2 antibody (ab52678)参考文献

ab52678 has not yet been referenced specifically in any publications.

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