The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Western Blot: Approx 65 kDa band seen in U937 lysate [Predicted MW of approx. 61kDa according to NP_005735].
Recommended for use at 0.2-1.0µg/ml.
Not tested in other applications.
Optimal working dilutions / concentrations should be determined by the end user.
Might act as an E3 ubiquitin-protein ligase, or as part of the E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates.
Belongs to the RBR family. Ariadne subfamily. Contains 1 IBR-type zinc finger. Contains 2 RING-type zinc fingers.
ab3891 staining (0.5µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane).Primary incubated for 1 hour.Detected by western blot using chemiluminescence. ab3891 staining (0.5µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.