The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 64 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Might act as an E3 ubiquitin-protein ligase, or as part of the E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates.
Belongs to the RBR family. Ariadne subfamily. Contains 1 IBR-type zinc finger. Contains 2 RING-type zinc fingers.
ab115300, at 5 µg/ml, staining ARIH1 in formalin-fixed, paraffin-embedded Human Adrenal tissue by Immunohistochemistry, using a biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-ARIH1 antibody (ab115300)
Anti-ARIH1 antibody (ab115300) at 0.5 µg/ml + U937 lysate (in RIPA buffer) at 30 µg Developed using the ECL technique