Anti-ARID5A抗体(ab81207)

概述

  • 产品名称Anti-ARID5A抗体
    参阅全部 ARID5A 一抗
  • 描述
    兔多克隆抗体to ARID5A
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide within amino acids 108-157 of Human ARID5A: RRLWKNVYDE LGGSPGSTSA ATCTRRHYER LVLPYVRHLK GEDDKPLPTS

  • 阳性对照
    • 721_B cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab81207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 64 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

靶标

  • 相关性ARID5A is a member of the ARID protein family, that have diverse functions but all appear to play important roles in development, tissue-specific gene expression, and regulation of cell growth.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • ARID domain containing protein 5A antibody
    • ARID-containing protein 5A antibody
    • AT rich interactive domain 5A antibody
    • AT rich interactive domain 5A (MRF1 like) antibody
    • AT rich interactive domain containing protein 5A antibody
    • Modulator recognition factor 1 antibody
    • MRF 1 antibody
    • MRF1 antibody
    • RFVG5814 antibody
    • RP11 363D14 antibody
    see all

Anti-ARID5A antibody 图像

  • Anti-ARID5A antibody (ab81207) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 64 kDa
    Observed band size : 64 kDa

Anti-ARID5A antibody (ab81207)参考文献

ab81207 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Mouse Cell lysate - whole cell (HEK 293 T cell line)
Specification HEK 293 T cell line
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Feb 24 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"