Anti-ARID2抗体[2036C1a] (ab51019)

概述

  • 产品名称Anti-ARID2抗体[2036C1a]
    参阅全部 ARID2 一抗
  • 描述
    小鼠单克隆抗体[2036C1a] to ARID2
  • 经测试应用适用于: WB, ICC, Dot blotmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment

  • 阳性对照
    • HeLa cells. HEK293 whole cell lysate. NIH3T3 whole cell lysate. F2408 whole cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane.
  • 克隆单克隆
  • 克隆编号2036C1a
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab51019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50. Detects a band of approximately 240 kDa (predicted molecular weight: 197 kDa).
ICC 1/5.
Dot blot Use at an assay dependent dilution.

靶标

  • 功能Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). May be involved in targeting the complex to different genes. May be involved in regulating transcriptional activation of cardiac genes.
  • 组织特异性Highly expressed in heart and testis.
  • 序列相似性Contains 1 ARID domain.
    Contains 1 C2H2-type zinc finger.
    Contains 1 RFX-type winged-helix DNA-binding domain.
  • 发展阶段Up-regulated in adult heart (at protein level).
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARID domain containing protein 2 antibody
    • ARID domain-containing protein 2 antibody
    • ARID2 antibody
    • ARID2_HUMAN antibody
    • AT rich interactive domain 2 (ARID, RFX like) antibody
    • AT rich interactive domain containing protein 2 antibody
    • AT-rich interactive domain-containing protein 2 antibody
    • BAF antibody
    • BAF200 antibody
    • BRG1 associated factor 200 antibody
    • BRG1-associated factor 200 antibody
    • DKFZp686G052 antibody
    • DKFZp779P0222 antibody
    • FLJ30619 antibody
    • KIAA1557 antibody
    • p200 antibody
    • Zinc finger protein with activation potential antibody
    • zip zap antibody
    • zipzap antibody
    • Zipzap/p200 antibody
    see all

Anti-ARID2 antibody [2036C1a] 图像

  • Anti-ARID2 antibody [2036C1a] (ab51019) at 1/50 dilution + HEK293 whole cell lysate at 25 µg

    Secondary
    Mouse IgG anibody at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 197 kDa
    Observed band size : 240 kDa (why is the actual band size different from the predicted?)
  • HeLa cells stained with ab51019 at 1/5 dilution, followed by Alexa Fluor 488 Goat Anti-Mouse IgG at 1/200 dilution.
  • Anti-ARID2 antibody [2036C1a] (ab51019) at 1/50 dilution + NIH3T3 whole cell lysate at 25 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 197 kDa
    Observed band size : 240 kDa (why is the actual band size different from the predicted?)
  • Anti-ARID2 antibody [2036C1a] (ab51019) at 1/50 dilution + F2408 whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 197 kDa
    Observed band size : 240 kDa (why is the actual band size different from the predicted?)

Anti-ARID2 antibody [2036C1a] (ab51019)参考文献

ab51019 has not yet been referenced specifically in any publications.

Product Wall

Thank you for forwarding the details of your protocol. The protocol looks fine, especially since you were able to detect a different protein of a similar size. I have credited your original order.

I am sorry that this antibody did not perfor...

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