概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to ARID1B
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: PPAKRHEGDM YNMQYSSQQQ EMYNQYGGSY SGPDRRPIQG QYPYPYSRER MQGPGQIQTH GIPPQMMGGP LQSSSSEGPQ QNMWAARNDM PYPYQNR, corresponding to amino acids 1364-1461 of Human ARID1B

  • 阳性对照
    • ICC/IF: HepG2 cells.
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

性能

应用

Our Abpromise guarantee covers the use of ab57461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 236 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 1-2µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性
    ARID1B (AT-rich interactive domain-containing protein 1B) is a component of SWI/SNF chromatin remodeling complexes involved in transcriptional activation and repression of select genes by chromatin remodeling. It binds DNA non-specifically and alters DNA-nucleosome topology.
  • 细胞定位
    Nuclear
  • 数据库链接
  • 别名
    • 6A3 5 antibody
    • ARID 1B antibody
    • ARID domain containing protein 1B antibody
    • AT rich interactive domain 1B (SWI1 like) antibody
    • AT rich interactive domain 1B antibody
    • AT rich interactive domain containing protein 1B antibody
    • BAF 250b antibody
    • BAF-associated factor, 250-KD, B antibody
    • BAF250b antibody
    • BRG1 associated factor 250b antibody
    • BRG1 binding protein antibody
    • BRG1 binding protein ELD/OSA1 antibody
    • BRG1 binding protein hELD/OSA1 antibody
    • BRIGHT antibody
    • DAN 15 antibody
    • DAN15 antibody
    • Eld (eyelid)/Osa protein antibody
    • ELD/OSA1 antibody
    • hELD/OSA1 antibody
    • hOsa 2 antibody
    • hOsa2 antibody
    • KIAA1235 antibody
    • MRD12 antibody
    • OSA 2 antibody
    • Osa homolog 2 antibody
    • OSA2 antibody
    • OTTHUMP00000040115 antibody
    • p250R antibody
    • RP11 419L10.1 antibody
    see all

图片

  • ICC/IF image of ab57461 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57461, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ARID1B antibody (ab57461) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human kidney.
  • ARID1B antibody (ab57461) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HEK293 cells stained with ab57461 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57461, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

文献

This product has been referenced in:
  • Wang B  et al. Expression of ARID1B Is Associated With Poor Outcomes and Predicts the Benefit from Adjuvant Chemotherapy in Bladder Urothelial Carcinoma. J Cancer 8:3490-3497 (2017). Read more (PubMed: 29151933) »
  • Shibutani M  et al. Arid1b Haploinsufficiency Causes Abnormal Brain Gene Expression and Autism-Related Behaviors in Mice. Int J Mol Sci 18:N/A (2017). Read more (PubMed: 28867767) »

See all 6 Publications for this product

客户评价及客户问答

Thank you for contacting us.

Your credit note ID is 23074.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in on...

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH8.5
Permeabilization
No
Specification
kidney
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 07 2015

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (JHH4)
Loading amount
10 µg
Specification
JHH4
Treatment
Use nuclear fraction or cytoplasmic fraction
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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