概述

  • 产品名称
    Anti-ARHGEF9抗体- C-terminal
    参阅全部 ARHGEF9 一抗
  • 描述
    兔多克隆抗体to ARHGEF9 - C-terminal
  • 经测试应用
    适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide from the C-terminal region of human ARHGEF9 conjugated to KLH

  • 阳性对照
    • 293 cell lysates transiently transfected with the ARHGEF9 gene. Human brain tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab71350 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab71350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
IHC-P 1/10 - 1/50.
WB 1/50 - 1/100. Detects a band of approximately 55 kDa (predicted molecular weight: 61 kDa).

靶标

  • 功能
    Acts as guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters.
  • 组织特异性
    Detected in brain. Detected at low levels in heart.
  • 疾病相关
    Defects in ARHGEF9 are a cause of startle disease with epilepsy (STHEE) [MIM:300607]; also known as hyperekplexia with epilepsy. Startle disease is a genetically heterogeneous neurologic disorder. STHE is characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile stimuli.
  • 序列相似性
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 9630036L12Rik antibody
    • A230067K14 antibody
    • ARHG9_HUMAN antibody
    • Arhgef9 antibody
    • Cdc42 guanine nucleotide exchange factor (GEF) 9 antibody
    • Collybistin antibody
    • collybistin I antibody
    • EIEE8 antibody
    • HPEM 2 antibody
    • hPEM-2 collybistin antibody
    • KIAA0424 antibody
    • mKIAA0424 antibody
    • OTTHUMP00000023424 antibody
    • OTTHUMP00000023425 antibody
    • OTTHUMP00000023427 antibody
    • PEM 2 antibody
    • PEM 2 homolog antibody
    • PEM-2 homolog antibody
    • PEM2 antibody
    • Rac/Cdc42 guanine nucleotide exchange factor 9 antibody
    • Rho guanine nucleotide exchange factor 9 antibody
    • RP11 357C3.4 antibody
    • RP23 230K20.3 antibody
    see all

图片

  • All lanes : Anti-ARHGEF9 antibody - C-terminal (ab71350) at 1/50 dilution

    Lane 1 : Nontransfected 293 cell lysates
    Lane 2 : 293 cell lysates transiently transfected with the ARHGEF9 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 61 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa (possible non-specific binding).
  • ab71350 at 1/10 dilution, staining ARHGEF9 in human brain by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

文献

This product has been referenced in:
  • Simon S  et al. Inter-kingdom Signaling by the Legionella Quorum Sensing Molecule LAI-1 Modulates Cell Migration through an IQGAP1-Cdc42-ARHGEF9-Dependent Pathway. PLoS Pathog 11:e1005307 (2015). Read more (PubMed: 26633832) »

See 1 Publication for this product

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