Anti-ARHGEF9抗体(ab87148)

概述

  • 产品名称
    Anti-ARHGEF9抗体
    参阅全部 ARHGEF9 一抗
  • 描述
    兔多克隆抗体to ARHGEF9
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human, Orangutan
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human ARHGEF9.

    (Peptide available as ab98294.)

  • 阳性对照
    • This antibody gave a positive signal in human brain and heart tissues lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab87148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).

靶标

  • 功能
    Acts as guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters.
  • 组织特异性
    Detected in brain. Detected at low levels in heart.
  • 疾病相关
    Defects in ARHGEF9 are a cause of startle disease with epilepsy (STHEE) [MIM:300607]; also known as hyperekplexia with epilepsy. Startle disease is a genetically heterogeneous neurologic disorder. STHE is characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile stimuli.
  • 序列相似性
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 9630036L12Rik antibody
    • A230067K14 antibody
    • ARHG9_HUMAN antibody
    • Arhgef9 antibody
    • Cdc42 guanine nucleotide exchange factor (GEF) 9 antibody
    • Collybistin antibody
    • collybistin I antibody
    • EIEE8 antibody
    • HPEM 2 antibody
    • hPEM-2 collybistin antibody
    • KIAA0424 antibody
    • mKIAA0424 antibody
    • OTTHUMP00000023424 antibody
    • OTTHUMP00000023425 antibody
    • OTTHUMP00000023427 antibody
    • PEM 2 antibody
    • PEM 2 homolog antibody
    • PEM-2 homolog antibody
    • PEM2 antibody
    • Rac/Cdc42 guanine nucleotide exchange factor 9 antibody
    • Rho guanine nucleotide exchange factor 9 antibody
    • RP11 357C3.4 antibody
    • RP23 230K20.3 antibody
    see all

图片

  • All lanes : Anti-ARHGEF9 antibody (ab87148) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 61 kDa


    Exposure time : 12 minutes

实验方案

文献

ab87148 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E13.5 mouse embryo (heart))
Permeabilization
No
Specification
E13.5 mouse embryo (heart)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Paraformaldehyde
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提交于 Feb 02 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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