The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IP: Use at 5-15 µg/mg of lysate.
Is unsuitable for WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Acts as a RAC1 guanine nucleotide exchange factor (GEF).
疾病相关Defects in ARHGEF6 are the cause of mental retardation X-linked type 46 (MRX46) [MIM:300436]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
Detection of ARHGEF6 by Western Blot of Immunprecipitate.
anti-ARHGEF6, at 1 µg/ml, staining ARHGEF6 in Jurkat whole cell lysate immunoprecipitated using ab91572 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
Anti-ARHGEF6 antibody (ab91572)参考文献
has not yet been referenced specifically in any publications.