The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 75 kDa).
This protein may form complexes with G proteins and stimulate Rho-dependent signals, and is similar to rat collybistin protein. Rho GTPases play a fundamental role in numerous cellular processes, which are initiated by extracellular stimuli that work through G protein coupled receptors. Alternative splicing of this gene generates two transcript variants which encode different isoforms.
Cytoplasm. Cell projection; ruffle membrane; Peripheral membrane protein; Cytoplasmic side Probable. Note: Associated with membrane ruffles