概述

  • 产品名称Anti-ARHGEF38抗体
  • 描述
    兔多克隆抗体to ARHGEF38
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence, corresponding to N terminal amino acids 1-77 (MEPKEATGKE NMVTKKKNLA FLRSRLYMLE RRKTDTVVES SVSGDHSGTL RRSQSDRTEY NQKLQEKMTP QGECSVA) of Human ARHGEF38

  • 阳性对照
    • Human skin tissue; Transfected HEK293T cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab122345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

靶标

Anti-ARHGEF38 antibody 图像

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in cytoskeleton (intermediate filaments). Recommended concentration of ab122345 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122345, at a 1/150 dilution, staining ARHGEF38 in paraffin-embedded Human skin tissue by immunohistochemistry.
  • All lanes : Anti-ARHGEF38 antibody (ab122345) at 1/250 dilution

    Lane 1 : Vector only transfected HEK293T lysate
    Lane 2 : ARHGEF38-transfected HEK293T over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))

    Developed using the ECL technique

Anti-ARHGEF38 antibody (ab122345)参考文献

ab122345 has not yet been referenced specifically in any publications.

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