antigen sequence, corresponding to N terminal amino acids 1-77 (MEPKEATGKE NMVTKKKNLA FLRSRLYMLE RRKTDTVVES SVSGDHSGTL RRSQSDRTEY NQKLQEKMTP QGECSVA) of Human ARHGEF38
- Human skin tissue; Transfected HEK293T cell lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
May act as a guanine-nucleotide releasing factor.
Contains 1 DH (DBL-homology) domain.
Information by UniProt
- ARH38_HUMAN antibody
- Arhgef38 antibody
- FLJ20184 antibody
- Rho guanine nucleotide exchange factor 38 antibody
Immunocytochemistry/ Immunofluorescence - Anti-ARHGEF38 antibody (ab122345)
Immunofluorescent staining of Human cell line U-251 MG shows positivity in cytoskeleton (intermediate filaments). Recommended concentration of ab122345 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGEF38 antibody (ab122345)
ab122345, at a 1/150 dilution, staining ARHGEF38 in paraffin-embedded Human skin tissue by immunohistochemistry.
Western blot - Anti-ARHGEF38 antibody (ab122345)
All lanes : Anti-ARHGEF38 antibody (ab122345) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T lysate
Lane 2 : ARHGEF38-transfected HEK293T over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"