antigen sequence: MNLGFGDESP EPEASGRGER LGRKVAPLAT TEDSLASIPF IDEPTSPSID LQAKHVPASA VVSSAMNSAP VLGTSPSS, corresponding to amino acids 509-586 of Human ARHGAP23.
Human cerebellum tissue; RT 4 and U 251 MG lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.
Expressed in placenta, prostate, hippocampus and brain medulla. Also expressed in brain tumor, salivary gland tumor, head and neck tumor.
Contains 1 PDZ (DHR) domain.
Contains 1 PH domain. Contains 1 Rho-GAP domain.
Information by UniProt
Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP23 antibody (ab121617)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and plasma membrane. Recommended concentration of ab121617 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP23 antibody (ab121617)
ab121617 at 1/125 staining ARHGAP23 in Paraffin Embedded Human cerebellum tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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