Anti-ARHGAP23抗体(ab121617)

概述

  • 产品名称Anti-ARHGAP23抗体
  • 描述
    兔多克隆抗体to ARHGAP23
  • 经测试应用适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: MNLGFGDESP EPEASGRGER LGRKVAPLAT TEDSLASIPF IDEPTSPSID LQAKHVPASA VVSSAMNSAP VLGTSPSS, corresponding to amino acids 509-586 of Human ARHGAP23.

  • 阳性对照
    • Human cerebellum tissue; RT 4 and U 251 MG lysates.

性能

应用

Our Abpromise guarantee covers the use of ab121617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.
  • 组织特异性Expressed in placenta, prostate, hippocampus and brain medulla. Also expressed in brain tumor, salivary gland tumor, head and neck tumor.
  • 序列相似性Contains 1 PDZ (DHR) domain.
    Contains 1 PH domain.
    Contains 1 Rho-GAP domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Arhgap23 antibody
    • KIAA1501 antibody
    • RHG23_HUMAN antibody
    • Rho GTPase-activating protein 23 antibody
    • Rho-type GTPase-activating protein 23 antibody
    see all

Anti-ARHGAP23 antibody 图像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and plasma membrane. Recommended concentration of ab121617 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121617 at 1/125 staining ARHGAP23 in Paraffin Embedded Human cerebellum tissue by Immunohistochemistry.

Anti-ARHGAP23 antibody (ab121617)参考文献

ab121617 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Rat Hippocampal Neurons)
Permeabilization Yes - 0.2% Triton-X 100 in PBS
Specification Rat Hippocampal Neurons
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 13 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"