Anti-Argonaute 4 with anti-GAPDH internal loading control抗体(ab94772)

概述

  • 产品名称Anti-Argonaute 4 with anti-GAPDH internal loading control抗体
  • 描述
    兔多克隆抗体to Argonaute 4 with anti-GAPDH internal loading control
  • 经测试应用适用于: WBmore details
    不适用于: ChIP,Flow Cyt,ICC/IF or IHC-P
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Argonaute 4 with anti-GAPDH internal loading control.

  • 阳性对照
    • This cocktail gave a positive signal in both Human Brain and Testis Tissue lysates as well as the following whole cell lysates: HeLa; A431; Jurkat; HepG2.

性能

应用

Our Abpromise guarantee covers the use of ab94772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37, 97 kDa (predicted molecular weight: 37, 97 kDa). ab94772 contains 2 antibodies, generated by raising against an Argonaute 4 immunogen and spiking with anti-GAPDH loading control.
  • 应用说明Is unsuitable for ChIP,Flow Cyt,ICC/IF or IHC-P.
  • 靶标

    • 功能Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for RNA-directed transcription and replication of the human hapatitis delta virus (HDV).
    • 序列相似性Belongs to the argonaute family. Ago subfamily.
      Contains 1 PAZ domain.
      Contains 1 Piwi domain.
    • 细胞定位Cytoplasm > P-body.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AGO4_HUMAN antibody
      • Argonaute4 antibody
      • eIF-2C 4 antibody
      • eIF2C 4 antibody
      • eif2c4 antibody
      • Eukaryotic translation initiation factor 2C 4 antibody
      • hAgo4 antibody
      • Protein argonaute-4 antibody
      see all

    Anti-Argonaute 4 with anti-GAPDH internal loading control antibody 图像

    • All lanes : Anti-Argonaute 4 with anti-GAPDH internal loading control antibody (ab94772) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Human brain tissue lysate - total protein (ab29466)
      Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 4 : Human testis tissue lysate - total protein (ab30257)
      Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 37, 97 kDa
      Observed band size : 37,97 kDa
      Additional bands at : 52 kDa,60 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 1 minute

    Anti-Argonaute 4 with anti-GAPDH internal loading control antibody (ab94772)参考文献

    ab94772 has not yet been referenced specifically in any publications.

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