概述

  • 产品名称
    Anti-Argonaute 4抗体
  • 描述
    兔多克隆抗体to Argonaute 4
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    A 15 amino acid synthetic peptide (Human) from near the N-terminus

  • 阳性对照
    • HeLa cell lysate

性能

  • 形式
    Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab85077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250 - 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
IHC-P 1/200.

靶标

  • 功能
    Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for RNA-directed transcription and replication of the human hapatitis delta virus (HDV).
  • 序列相似性
    Belongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • 细胞定位
    Cytoplasm > P-body.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5730550L01Rik antibody
    • Ago 4 antibody
    • Ago4 antibody
    • AGO4_HUMAN antibody
    • Argonaute4 antibody
    • EIF 2C 4 antibody
    • eIF-2C 4 antibody
    • eIF2C 4 antibody
    • eif2c4 antibody
    • Eukaryotic translation initiation factor 2C 4 antibody
    • eukaryotic translation initiation factor 2C antibody
    • hAgo4 antibody
    • KIAA1567 antibody
    • Piwi/Argonaute family protein meIF2C4 antibody
    • Protein argonaute-4 antibody
    see all

图片

  • Anti-Argonaute 4 antibody (ab85077) at 1/500 dilution + HeLa cell lysate

    Predicted band size: 97 kDa
    Observed band size: 97 kDa

  • ab85077 staining Argonaute 4 in Mouse testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/200 in PBS + casein) for 1 hour 30 mins at 37°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Yu D  et al. Multiple microRNAs function as self-protective modules in acetaminophen-induced hepatotoxicity in humans. Arch Toxicol 92:845-858 (2018). EMSA ; Human . Read more (PubMed: 29067470) »
  • Voutila J  et al. Development and Mechanism of Small Activating RNA Targeting CEBPA, a Novel Therapeutic in Clinical Trials for Liver Cancer. Mol Ther 25:2705-2714 (2017). Read more (PubMed: 28882451) »

See all 3 Publications for this product

客户评价及客户问答

Thank you for your enquiry. To date, neither of our anti-AGO4 antibodies have been tested in IP. Both ab85077 and ab52724 have thusfar only been tested in western blotting. They may be useful for IP but we do not have any data to suggest it. ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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