Anti-Argonaute 4抗体(ab85077)


  • 产品名称Anti-Argonaute 4抗体
  • 描述
    兔多克隆抗体to Argonaute 4
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    A 15 amino acid synthetic peptide (Human) from near the N-terminus

  • 阳性对照
    • HeLa cell lysate


  • 形式Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab85077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250 - 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
IHC-P 1/200.


  • 功能Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for RNA-directed transcription and replication of the human hapatitis delta virus (HDV).
  • 序列相似性Belongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • 细胞定位Cytoplasm > P-body.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5730550L01Rik antibody
    • Ago 4 antibody
    • Ago4 antibody
    • AGO4_HUMAN antibody
    • Argonaute4 antibody
    • EIF 2C 4 antibody
    • eIF-2C 4 antibody
    • eIF2C 4 antibody
    • eif2c4 antibody
    • Eukaryotic translation initiation factor 2C 4 antibody
    • eukaryotic translation initiation factor 2C antibody
    • hAgo4 antibody
    • KIAA1567 antibody
    • Piwi/Argonaute family protein meIF2C4 antibody
    • Protein argonaute-4 antibody
    see all

Anti-Argonaute 4 antibody 图像

  • Anti-Argonaute 4 antibody (ab85077) at 1/500 dilution + HeLa cell lysate

    Predicted band size : 97 kDa
    Observed band size : 97 kDa
  • ab85077 staining Argonaute 4 in Mouse testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/200 in PBS + casein) for 1 hour 30 mins at 37°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

Anti-Argonaute 4 antibody (ab85077)参考文献

ab85077 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (testis)
Specification testis
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Apr 11 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application CLIP
Sample Human Cell lysate - whole cell (HEK 293T)
Specification HEK 293T
Type iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control Anti-hnRNP C antibody (sc-15386) bound protein A dynabeads, 0.1 mg/ml (antibody/beads)
Negative control Protein A dynabeads without antibody bound
Immuno-precipitation step Other - Protein A dynabeads

Abcam user community

Verified customer

提交于 Mar 21 2012

Thank you for your enquiry. To date, neither of our anti-AGO4 antibodies have been tested in IP. Both ab85077 and ab52724 have thusfar only been tested in western blotting. They may be useful for IP but we do not have any data to suggest it. ...

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