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Synthetic peptide corresponding to Drosophila melanogaster Argonaute-2 aa 550-650 conjugated to keyhole limpet haemocyanin.
(Peptide available as
Our Abpromise guarantee covers the use of ab5072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 55, 95 kDa (predicted molecular weight: 106 kDa).
Detects a band of approximately 55 + 95 kDa (predicted molecular weight: 106 kDa). Swissprot suggests Ago 2 may have 50kDa form. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab5072 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab5072 staining Ago2 / eIF2C2 (green) in fruit fly (Drosophilia melanogaster) OSS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Tween and blocked with 1% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG monoclonal (1/5000) was used as the secondary antibody.
ab24609 was used to stain Nuclear Pore Complex Proteins (red).
Rabbit polyclonal to Ago2 (ab5072) at 1/500 on Drosophila lysate (20ug).
Detects a band of approximately 55 + 95 kDa (predicted molecular weight: 106 kDa). Swissprot suggests Ago 2 may have 50kDa form.
Secondary antibody - Goat polyclonal to rabbit IgG (HRP) - ab6721.Swissprot suggests Ago 2 may have 50kDa form.
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