Anti-Argonaute-2抗体[2E12-1C9] - ChIP Grade (ab57113)

概述

  • 产品名称
    Anti-Argonaute-2抗体[2E12-1C9] - ChIP Grade
    参阅全部 Argonaute-2 一抗
  • 描述
    小鼠单克隆抗体[2E12-1C9] to Argonaute-2 - ChIP Grade
  • 经测试应用
    适用于: CHIPseq, WB, ICC/IF, IHC-P, IP, Flow Cyt, ChIP, ICCmore details
  • 种属反应性
    与反应: Chicken, Human, Zebrafish
    不与反应: Xenopus laevis
  • 免疫原

    Recombinant fragment corresponding to Human Argonaute-2 aa 483-859.

性能

应用

Our Abpromise guarantee covers the use of ab57113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
CHIPseq Use at an assay dependent concentration. PubMed: 23592263
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 97 kDa.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 3 µg/ml.
IP Use at an assay dependent concentration. PubMed: 19574298
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ChIP Use at an assay dependent concentration. PubMed: 20010811
ICC Use at an assay dependent concentration.

靶标

  • 功能
    Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by EIF2C2/AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also upregulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and upregulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
  • 序列相似性
    Belongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • 结构域
    The Piwi domain may perform RNA cleavage by a mechanism similar to that of RNase H. However while RNase H utilizes a triad of Asp-Asp-Glu (DDE) for metal ion coordination, this protein appears to utilize a triad of Asp-Asp-His (DDH).
  • 翻译后修饰
    Hydroxylated. 4-hydroxylation appears to enhance protein stability but is not required for miRNA-binding or endonuclease activity.
  • 细胞定位
    Cytoplasm > P-body. Nucleus. Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ago 2 antibody
    • AGO2_HUMAN antibody
    • Argonaute 2 antibody
    • argonaute 2, RISC catalytic component antibody
    • Argonaute RISC catalytic component 2 antibody
    • Argonaute2 antibody
    • CTA-204B4.6 antibody
    • dAgo2 antibody
    • eIF 2C 2 antibody
    • eIF-2C 2 antibody
    • eIF2C 2 antibody
    • Eif2c2 antibody
    • Eukaryotic translation initiation factor 2C 2 antibody
    • Eukaryotic translation initiation factor 2C subunit 2 antibody
    • hAgo2 antibody
    • MGC3183 antibody
    • PAZ Piwi domain protein antibody
    • PPD antibody
    • Protein argonaute-2 antibody
    • Protein slicer antibody
    • Q10 antibody
    • Slicer protein antibody
    see all

图片



  • Predicted band size : 97 kDa
    Western Blot detection against the recombinant fragment imunogen (68.4 KDa).
  • Ago2 / eIF2C2 antibody (ab57113) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach.

  • Ago2 / eIF2C2 antibody (ab57113) used in immunofluorescence at 10ug/ml on HeLa cells.

  • Overlay histogram showing HeLa cells stained with ab57113 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57113, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

文献

This product has been referenced in:
  • Özata DM  et al. Loss of miR-514a-3p regulation of PEG3 activates the NF-kappa B pathway in human testicular germ cell tumors. Cell Death Dis 8:e2759 (2017). WB, IP ; Human . Read more (PubMed: 28471449) »
  • Beltrami C  et al. Human Pericardial Fluid Contains Exosomes Enriched with Cardiovascular-Expressed MicroRNAs and Promotes Therapeutic Angiogenesis. Mol Ther 25:679-693 (2017). Read more (PubMed: 28159509) »

See all 70 Publications for this product

客户评价及客户问答

Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Human Cell lysate - other (Cytoplasmic extract, A549 cells)
Specification
Cytoplasmic extract, A549 cells
Total protein in input
1000 µg
Username

Abcam user community

Verified customer

提交于 Feb 19 2015

Application
Western blot
Loading amount
20 cells
Gel Running Conditions
Reduced Denaturing
Sample
Xenopus laevis Cell lysate - whole cell (oocytes)
Specification
oocytes
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Nov 21 2013

I can confirm that after checking with some colleagues, the cross reactivity of ab57113 with rabbit IgG has not been tested yet. Therefore, we have no related data to share with you at this moment.

Unfortunately, we do not have any antibodies against Anti-ElF2C2 (hAgo2) which are directly conjugated to a fluorochrome available in our catalog.

We do however have kits which can be used to conjugate your primary antibody with a fluorochrom...

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1.
Our data for ab57113 on zebrafish samples comes from an abreview submitted by a customer on 23 March 2011 showing positive results in western blot. These results can be found at the following webpage:
http://www.abcam.com/Ago2--eIF2C2-anti...

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Thank you for contacting us in regards to the Anti-Ago2 / eIF2C2 antibody - ChIP Grade (ab57113) and I am sorry for the delay in getting back to you.

I now have further information to share with you. The anti-Ago2 / eIF2C2 antibody - ChIP Grad...

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Thank you for your email.

I can confirm that the 6 units of ab57113 were added to your order. You will get 5 units of lot GB90870-1 from the UK, one unit with lot GB90870-1 will be requested from the US.

I hope you will find everythin...

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Thank you for contacting me again.

At the moment we have five units of ab57113 with the same lot. The currently available lot number is GR90870-1. If you like to order the product you should send your order as soon as possible and include the ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (HELA)
Specification
HELA
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X in PBS
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Oct 23 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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