Anti-Argininosuccinate Lyase抗体(ab97370)

概述

  • 产品名称Anti-Argininosuccinate Lyase抗体
    参阅全部 Argininosuccinate Lyase 一抗
  • 描述
    兔多克隆抗体to Argininosuccinate Lyase
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 13 - 231 of Human Argininosuccinate Lyase (AAH08195).

  • 阳性对照
    • WB: 293T, A431, H1299, HeLa, HepG2 and MOLT4 cell lysates IF: HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab97370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
ICC/IF 1/100 - 1/200.

靶标

  • 通路Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.
    Nitrogen metabolism; urea cycle; L-arginine and fumarate from (N(omega)-L-arginino)succinate: step 1/1.
  • 疾病相关Defects in ASL are the cause of arginosuccinicaciduria (ARGINSA) [MIM:207900]. Arginosuccinicaciduria is an autosomal recessive disorder of the urea cycle. The disease is characterized by mental and physical retardation, liver enlargement, skin lesions, dry and brittle hair showing trichorrhexis nodosa microscopically and fluorescing red, convulsions, and episodic unconsciousness.
  • 序列相似性Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.
  • 翻译后修饰Acetylation modifies enzyme activity in response to alterations of extracellular nutrient availability. Acetylation increased with trichostin A (TSA) or with nicotinamide (NAM). Glucose increases acetylation by about a factor of 3 with decreasing enzyme activity. Acetylation on Lys-288 is decreased on the addition of extra amino acids resulting in activation of enzyme activity.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Argininosuccinase antibody
    • Argininosuccinate lyase antibody
    • Arginosuccinase antibody
    • ARLY_HUMAN antibody
    • ASAL antibody
    • ASL antibody
    • EC 4.3.2.1 antibody
    see all

Anti-Argininosuccinate Lyase antibody 图像

  • All lanes : Anti-Argininosuccinate Lyase antibody (ab97370) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 52 kDa
  • ab97370, at a 1/200 dilution, staining Argininosuccinate Lyase in paraformaldehyde-fixed HeLa cells by Immunofluorescence analysis. Right image is merged with a DNA probe.

Anti-Argininosuccinate Lyase antibody (ab97370)参考文献

ab97370 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A375)
Loading amount 20 µg
Specification A375
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Jan 21 2011

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