概述

  • 产品名称Anti-Arg 3.1抗体
    参阅全部 Arg 3.1 一抗
  • 描述
    兔多克隆抗体to Arg 3.1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Goat, Chicken, Guinea pig, Cow
  • 免疫原

    Synthetic peptide: LDLPQKQGEP LDQFLWRKRD LYQTLYVDAD EEEIIQYVVG TLQPKLKRFL , corresponding to internal sequence amino acids 288-337 of Human Arg 3.1 (NP_056008).

  • 阳性对照
    • Transfected 293T cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab85656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa.Can be blocked with Arg 3.1 peptide (ab117435). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Required for consolidation of synaptic plasticity as well as formation of long-term memory. Regulates endocytosis of AMPA receptors in response to synaptic activity. Required for homeostatic synaptic scaling of AMPA receptors (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration.
  • 序列相似性Belongs to the ARC/ARG3.1 family.
  • 细胞定位Cytoplasm, cytoskeleton. Endosome. Cytoplasmic vesicle, secretory vesicle, acrosome. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, dendrite. Cell projection, dendritic spine. Cell junction, synapse. Associated with the cell cortex of neuronal soma and dendrites. Enriched in postsynaptic density of dendritic spines. Associated with the sperm tail (By similarity). Enriched on the plasma membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activity regulated cytoskeletal associated protein antibody
    • Activity regulated cytoskeletal protein antibody
    • Activity regulated gene 3.1 protein homolog antibody
    • Activity-regulated cytoskeleton-associated protein antibody
    • Activity-regulated gene 3.1 protein homolog antibody
    • Arc antibody
    • ARC/ARG3.1 antibody
    • ARC_HUMAN antibody
    • Arg3.1 antibody
    • KIAA0278 antibody
    see all

Anti-Arg 3.1 antibody 图像

  • Anti-Arg 3.1 antibody (ab85656) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
  • IHC image of ab85656 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85656, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human adrenal tissue labelling Arg 3.1 with ab85656 at 5µg/ml.

Anti-Arg 3.1 antibody (ab85656)参考文献

This product has been referenced in:
  • Gilbert MT & Soderstrom K Novel song-stimulated dendritic spine formation and Arc/Arg3.1 expression in zebra finch auditory telencephalon are disrupted by cannabinoid agonism. Brain Res 1541:9-21 (2013). Read more (PubMed: 24134952) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. I was able to have the blocking peptide for ab85656 added to our catalog as ab117435. I hope this helps, please let me know if you need any additional assistance.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"