概述

  • 产品名称
    Anti-Arg 3.1抗体
    参阅全部 Arg 3.1 一抗
  • 描述
    兔多克隆抗体to Arg 3.1
  • 经测试应用
    适用于: IHC-Fr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat Arg3.1.

    (Peptide available as ab26899.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Mouse forebrain, Mouse hippocampus, Rat cortex, Rat hippocampus.

性能

应用

Our Abpromise guarantee covers the use of ab23382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/300.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 45 kDa).

Abcam recommends using 3% milk as the blocking agent.

靶标

  • 功能
    Required for consolidation of synaptic plasticity as well as formation of long-term memory. Regulates endocytosis of AMPA receptors in response to synaptic activity. Required for homeostatic synaptic scaling of AMPA receptors (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration.
  • 序列相似性
    Belongs to the ARC/ARG3.1 family.
  • 细胞定位
    Cytoplasm, cytoskeleton. Endosome. Cytoplasmic vesicle, secretory vesicle, acrosome. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, dendrite. Cell projection, dendritic spine. Cell junction, synapse. Associated with the cell cortex of neuronal soma and dendrites. Enriched in postsynaptic density of dendritic spines. Associated with the sperm tail (By similarity). Enriched on the plasma membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activity regulated cytoskeletal associated protein antibody
    • Activity regulated cytoskeletal protein antibody
    • Activity regulated gene 3.1 protein homolog antibody
    • Activity-regulated cytoskeleton-associated protein antibody
    • Activity-regulated gene 3.1 protein homolog antibody
    • Arc antibody
    • ARC/ARG3.1 antibody
    • ARC_HUMAN antibody
    • Arg3.1 antibody
    • KIAA0278 antibody
    see all

Anti-Arg 3.1 antibody 图像

  • All lanes : Anti-Arg 3.1 antibody (ab23382) at 1 µg/ml (with 3% milk block)

    Lane 1 : Forebrain (Mouse) Tissue Lysate
    Lane 2 : Rat Cortex Tissue Lysate
    Lane 3 : Hippocampus (Mouse) Tissue Lysate
    Lane 4 : Rat Hippocampus Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 12 minutes
  • All lanes : Anti-Arg 3.1 antibody (ab23382) at 1 µg/ml

    Lane 1 : Hippocampus (Mouse) Tissue Lysate blocked with 5% BSA
    Lane 2 : Rat Hippocampus Tissue Lysate blocked with 5% BSA
    Lane 3 : Hippocampus (Mouse) Tissue Lysate blocked with 5% milk
    Lane 4 : Rat Hippocampus Tissue Lysate blocked with 5% milk
    Lane 5 : Hippocampus (Mouse) Tissue Lysate blocked with 3% milk
    Lane 6 : Rat Hippocampus Tissue Lysate blocked with 3% milk
    Lane 7 : Hippocampus (Mouse) Tissue Lysate blocked with 1% milk
    Lane 8 : Rat Hippocampus Tissue Lysate blocked with 1% milk

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

Anti-Arg 3.1 antibody (ab23382)参考文献

This product has been referenced in:
  • Wu H  et al. Salicylate-induced changes in immediate-early genes in the hippocampal CA1 area. Mol Med Rep 12:1625-30 (2015). WB, IHC-P ; Rat . Read more (PubMed: 25873216) »

See 1 Publication for this product

Product Wall

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8% Bis-Tris in MES)
Sample
Mouse Tissue lysate - other (Hippocampal Cytoplasm)
Specification
Hippocampal Cytoplasm
Blocking step
Li-Cor Block Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
Username

Grant Corbett

Verified customer

提交于 Jul 23 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Spinal cord)
Specification
Spinal cord
Fixative
Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

提交于 Dec 10 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (e14.5 striatum)
Specification
e14.5 striatum
Fixative
Paraformaldehyde
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Dean Griffiths

Verified customer

提交于 Aug 17 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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