Anti-ARFGAP1抗体(ab85971)
Key features and details
- Rabbit polyclonal to ARFGAP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ARFGAP1抗体 -
描述
兔多克隆抗体to ARFGAP1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human ARFGAP1 aa 466-516.
Database link: NP_055385.2 -
阳性对照
- HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab85971 was affinity purified using an epitope specific to ARFGAP1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85971于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes. -
组织特异性
Widely expressed. Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary. -
序列相似性
Contains 1 Arf-GAP domain. -
发展阶段
Expressed at higher level in adult thymus, brain and lung, than in corresponding fetal tissues. Expressed at lower level in spleen, heart, kidney and liver during development. -
细胞定位
Cytoplasm. Golgi apparatus membrane. Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment. - Information by UniProt
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数据库链接
- Entrez Gene: 458885 Chimpanzee
- Entrez Gene: 26286 Human
- Entrez Gene: 711160 Rhesus monkey
- Omim: 612439 Human
- SwissProt: Q9NP61 Human
- SwissProt: Q5R787 Orangutan
- Unigene: 685225 Human
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别名
- ADP ribosylation factor GTPase activating protein 1 antibody
- ADP ribosylation factor GTPase activating protein 3 antibody
- ADP-ribosylation factor GTPase-activating protein 3 antibody
see all
图片
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All lanes : Anti-ARFGAP1 antibody (ab85971) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 57 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Detection of ARFGAP1 by Western Blot of Immunprecipitate.
ab85971 at 1µg/ml staining ARFGAP1 in HeLa whole cell lysate immunoprecipitated using ab85971 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab85971 被引用在 1 文献中.
- Anthony DF et al. {beta}-Arrestin 1 Inhibits the GTPase-Activating Protein Function of ARHGAP21, Promoting Activation of RhoA following Angiotensin II Type 1A Receptor Stimulation. Mol Cell Biol 31:1066-75 (2011). WB ; Human . PubMed: 21173159