Anti-ARFGAP3抗体(ab27959)

概述

  • 产品名称
    Anti-ARFGAP3抗体
    参阅全部 ARFGAP3 一抗
  • 描述
    山羊多克隆抗体to ARFGAP3
  • 特异性
    Ab27959 recognises ARFGAP1.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chicken, Cynomolgus monkey
  • 免疫原

    Synthetic peptide:

    NGVVTSIQDRYGS

    , corresponding to C terminal amino acids 504-516 of Human ARFGAP1.

  • 阳性对照
    • Mouse brain extract.

性能

应用

Our Abpromise guarantee covers the use of ab27959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 44 kDa.

靶标

  • 功能
    GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
  • 组织特异性
    Widely expressed. Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary.
  • 序列相似性
    Contains 1 Arf-GAP domain.
  • 发展阶段
    Expressed at higher level in adult thymus, brain and lung, than in corresponding fetal tissues. Expressed at lower level in spleen, heart, kidney and liver during development.
  • 细胞定位
    Cytoplasm. Golgi apparatus membrane. Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADP ribosylation factor GTPase activating protein 1 antibody
    • ADP ribosylation factor GTPase activating protein 3 antibody
    • ADP-ribosylation factor GTPase-activating protein 3 antibody
    • ARF GAP 3 antibody
    • ARFG3_HUMAN antibody
    • ArfGAP 3 antibody
    • ARFGAP1 antibody
    • ARFGAP3 antibody
    • FLJ45618 antibody
    see all

图片

  • All lanes : Anti-ARFGAP3 antibody (ab27959) at 1/1000 dilution

    Lane 1 : 50000 non-transfected HeLa cells.
    Lane 2 : 50000 HeLa cells expressing closely related ArfGAP2 (zfp289) in the pPCMUIV vector.
    Lane 3 : 50000 HeLa cells transfected with full length ArfGAP3 in the pPCMUIV vector.

    Secondary
    HRP conjugated rabbit anti-goat IgG
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    This image is courtesy of an Abreview submitted by Mr Fredrik Kartberg

    See Abreview

实验方案

文献

ab27959 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
50000 cells
Specification
HeLa cells
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 5%
Username

Mr. Fredrik Kartberg

Verified customer

提交于 Sep 13 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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