Anti-ARFGAP3抗体(ab27959)

概述

  • 产品名称Anti-ARFGAP3抗体
    参阅全部 ARFGAP3 一抗
  • 描述
    山羊多克隆抗体to ARFGAP3
  • 特异性Ab27959 recognises ARFGAP1.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chicken, Cynomolgus Monkey
  • 免疫原

    Synthetic peptide:

    NGVVTSIQDRYGS

    , corresponding to C terminal amino acids 504-516 of Human ARFGAP1.

  • 阳性对照
    • Mouse brain extract.

性能

应用

Our Abpromise guarantee covers the use of ab27959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 44 kDa.

靶标

  • 功能GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
  • 组织特异性Widely expressed. Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary.
  • 序列相似性Contains 1 Arf-GAP domain.
  • 发展阶段Expressed at higher level in adult thymus, brain and lung, than in corresponding fetal tissues. Expressed at lower level in spleen, heart, kidney and liver during development.
  • 细胞定位Cytoplasm. Golgi apparatus membrane. Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADP ribosylation factor GTPase activating protein 1 antibody
    • ADP ribosylation factor GTPase activating protein 3 antibody
    • ADP-ribosylation factor GTPase-activating protein 3 antibody
    • ARF GAP 3 antibody
    • ARFG3_HUMAN antibody
    • ArfGAP 3 antibody
    • ARFGAP1 antibody
    • ARFGAP3 antibody
    • FLJ45618 antibody
    see all

Anti-ARFGAP3 antibody 图像

  • All lanes : Anti-ARFGAP3 antibody (ab27959) at 1/1000 dilution

    Lane 1 : 50000 non-transfected HeLa cells.
    Lane 2 : 50000 HeLa cells expressing closely related ArfGAP2 (zfp289) in the pPCMUIV vector.
    Lane 3 : 50000 HeLa cells transfected with full length ArfGAP3 in the pPCMUIV vector.

    Secondary
    HRP conjugated rabbit anti-goat IgG
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    This image is courtesy of an Abreview submitted by Mr Fredrik Kartberg

    See Abreview

Anti-ARFGAP3 antibody (ab27959)参考文献

ab27959 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 50000 cells
Specification HeLa cells
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 5%
Username

Mr. Fredrik Kartberg

Verified customer

提交于 Sep 13 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"