概述

  • 产品名称Anti-ARF6抗体
    参阅全部 ARF6 一抗
  • 描述
    兔多克隆抗体to ARF6
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 110-159 (REMRDAIILI FANKQDLPDA MKPHEIQEKL GLTRIRDRNW YVQPSCATSG) of human ARF6 (NP_001654).

  • 阳性对照
    • MCF7 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified by peptide affinity chromatography method.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:62500.

靶标

Anti-ARF6 antibody 图像

  • Anti-ARF6 antibody (ab81650) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 20 kDa
    Observed band size : <22 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry of paraffin embedded brain, cerebellum tissue, tested with an antibody dilution of 5 µg/ml (ab81650)

Anti-ARF6 antibody (ab81650)参考文献

This product has been referenced in:
  • Humphreys D  et al. Arf6 coordinates actin assembly through the WAVE complex, a mechanism usurped by Salmonella to invade host cells. Proc Natl Acad Sci U S A 110:16880-5 (2013). WB ; Human . Read more (PubMed: 24085844) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"