概述

  • 产品名称Anti-ARF1抗体[EP442Y]
    参阅全部 ARF1 一抗
  • 描述
    兔单克隆抗体[EP442Y] to ARF1
  • 特异性This antibody recognises ARF1 (small GTP-binding ADP-ribosylation factor 1). It is predicted to detect splice isoforms 3, 4, 5 based on sequence analysis.
  • 经测试应用适用于: WB, IHC-P, Flow Cytmore details
    不适用于: ICC or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in human ARF1.

  • 阳性对照
    • HEK-293 cell lysates and human breast carcinoma tissue.
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab32524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa).
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for ICC or IP.
  • 靶标

    • 功能GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
    • 序列相似性Belongs to the small GTPase superfamily. Arf family.
    • 细胞定位Golgi apparatus. Cytoplasm > perinuclear region.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ADP Ribosylation Factor 1 antibody
      • ADP-ribosylation factor 1 antibody
      • ARF 1 antibody
      • ARF1 antibody
      • ARF1_HUMAN antibody
      see all

    Anti-ARF1 antibody [EP442Y] 图像

    • Immunofluorescence staining of MCF7 cells with purified ab32524 working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 100% methanol. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

    • Anti-ARF1 antibody [EP442Y] (ab32524) at 1/2000 dilution + HEK-293 cell lysate

      Predicted band size : 21 kDa
      Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab32524 at 1/250 dilution.
    • Overlay histogram showing HeLa cells stained with ab32524 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32524, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Anti-ARF1 antibody [EP442Y] (ab32524)参考文献

    This product has been referenced in:
    • Covington JD  et al. Skeletal muscle perilipin 3 and coatomer proteins are increased following exercise and are associated with fat oxidation. PLoS One 9:e91675 (2014). WB ; Human . Read more (PubMed: 24632837) »
    • Schultz ML  et al. CLN3 deficient cells display defects in the ARF1-Cdc42 pathway and actin-dependent events. PLoS One 9:e96647 (2014). WB ; Mouse . Read more (PubMed: 24792215) »

    See all 6 Publications for this product

    Product Wall

    Thank you for your enquiry.

    I contacted the laboratories regarding the unsuitability of this antibody for ICC-IF. When it was tested, it showed incorrect staining localization. It worked well in the other applications listed on the datasheet....

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    Thank you for your enquiry. The peptide sequence for this product is:CNTQGLIFVVDSNDRER I am sorry but we do not have any information on whether this product will also detect other ARFs but I can confirm that this antibody recognizes ARF1 (small ...

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    Thank you for your enquiry. This antibody should work very well on your HEK293 cells, since this cell type has been shown to express this protein at detectable amounts. Since this cell type is listed as the positive control, you should be able to s...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"