Anti-ARF1抗体(ab76082)

概述

  • 产品名称Anti-ARF1抗体
    参阅全部 ARF1 一抗
  • 描述
    兔多克隆抗体to ARF1
  • 特异性ab76082 does not cross react to other members of the Ras family or to other ARF members. This antibody will label ARF 1 isoform 1, 2, 3 and 4.
  • 经测试应用适用于: IP, ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to a unique epitope on Rat ARF1 protein. The peptide was covalently modified post-synthetically to achieve desired antigenicity.

性能

应用

Our Abpromise guarantee covers the use of ab76082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB 1/500. Predicted molecular weight: 21 kDa.
ELISA 1/10000.

靶标

  • 功能GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
  • 序列相似性Belongs to the small GTPase superfamily. Arf family.
  • 细胞定位Golgi apparatus. Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADP Ribosylation Factor 1 antibody
    • ADP-ribosylation factor 1 antibody
    • ARF 1 antibody
    • ARF1 antibody
    • ARF1_HUMAN antibody
    see all

Anti-ARF1 antibody 图像

  • ab76082 staining ARF1 in human neuroblastoma cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized for 15 minutes in 0.01% Triton X-100, blocked using 5% serum for 30 minutes at 20°C, then incubated with ab76082 at a 1/100 dilution for 2 hours at 20°C. The secondary used was a Dylight 488 conjugated donkey anti-rabbit IgG (H+L) used at a 1/500 dilution.

    See Abreview

Anti-ARF1 antibody (ab76082)参考文献

ab76082 has not yet been referenced specifically in any publications.

Product Wall

This product was tested with rat, human and mouse samples only; Saccharomyces was never tried. The rat and S. cerevisiae proteins shows 78% homology http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I201306...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Rat Tissue lysate - whole (Brain)
Total protein in input 1000 µg
Specification Brain
Immuno-precipitation step Protein G
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提交于 Sep 04 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Neuroblastoma)
Specification Neuroblastoma
Fixative Paraformaldehyde
Permeabilization Yes - 15 mins in 0.01% Triton X
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Sep 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"