Anti-ARF 1+3抗体(ab91169)

概述

  • 产品名称Anti-ARF 1+3抗体
  • 描述
    兔多克隆抗体to ARF 1+3
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    KLH-conjugated synthetic peptide selected from the Center region of Human ARF 1+3.

  • 阳性对照
    • MCF-7 cell line lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Ammonium Sulphate Precipitation
  • 纯化说明This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab91169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Predicted molecular weight: 21 kDa.
ELISA 1/1000.

靶标

  • 相关性ADP ribosylation factors (ARFs)1 are small G proteins of the Ras superfamily involved in intracellular trafficking. ARF1 functions in the formation of transport vesicles. ARF1 controls the binding to Golgi membranes of coatomer, a large (700 kDa) cytoplasmic complex, which upon oligomerization deforms the lipid bilayer and induces the formation of "COPI" coated vesicles. In addition, ARF1 activates phospholipase D. ARF1 may recruit coatomer to the membrane based on the ability of ARF1 to interact directly, simultaneously, and in a GTP dependent manner both with its protein target and with lipid membranes. This dual interaction can be ascribed to two regions of ARF1. The classical switch I and II regions of the "Raslike" core domain of ARF1 are probably the main determinants of its interaction with coatomer, whereas the interaction of ARF1 with membrane lipids involves a region specific to ARF1: the N terminal helix, which is amphipathic and myristoylated. Three human ARF genes have been isolated that share high homology: 96% between ARF1 and 3; 84% between both ARF3 and 4 and ARF 1 and 4. There is consistency between ARFs between species.
  • 细胞定位Golgi Apparatus
  • 数据库链接
  • 别名
    • ADP ribosylation factor 1 antibody
    • ADP ribosylation factor 3 antibody
    • ARF1 antibody
    • ARF3 antibody

Anti-ARF 1+3 antibody 图像

  • Anti-ARF 1+3 antibody (ab91169) at 1/50 dilution + MCF-7 cell line lysate at 35 µg

    Predicted band size : 21 kDa

Anti-ARF 1+3 antibody (ab91169)参考文献

ab91169 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (kidney, epithelial cells)
Loading amount 100000 cells
Specification kidney, epithelial cells
Treatment transfected (or not) with ARF3-HA and infected (or not) with influenza A virus
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 18°C
Username

Dr. Maria Amorim

Verified customer

提交于 Apr 05 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (kidney, epithelial cells)
Specification kidney, epithelial cells
Fixative Formaldehyde
Permeabilization Yes - 0.2% triton x100 in PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 18°C
Username

Dr. Maria Amorim

Verified customer

提交于 Apr 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Panc-1 cell)
Loading amount 60 µg
Specification Panc-1 cell
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Sep 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"