概述

  • 产品名称
  • 描述
    山羊多克隆抗体to ARAP3
  • 宿主
    Goat
  • 经测试应用
    适用于: IHC-P, IP, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide:

    CTSSPPSSQPLT

    , corresponding to C terminal amino acids 1533-1544 of Human ARAP3.

  • 常规说明
    GenBank Accession Number – NP_071926

性能

应用

Our Abpromise guarantee covers the use of ab5911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent dilution. PubMed: 21076469
IP Use at an assay dependent dilution. PubMed: 21076469
WB Use at an assay dependent dilution. Can be blocked with Human ARAP3 peptide (ab23118). PubMed: 21076469

靶标

  • 相关性
    The ARAP3 gene encodes a phosphoinositide binding protein containing ARF-GAP, RHO-GAP, RAS-associating, and pleckstrin homology domains. The ARF-GAP and RHO-GAP domains cooperate in mediating rearrangements in the cell cytoskeleton and cell shape.
  • 数据库链接
  • 别名
    • Ankyrin repeat and plekstrin homology domains containing protein 3 antibody
    • ARAP 3 antibody
    • ARF GAP antibody
    • ARF-GAP RHO-GAP ankyrin repeat and plekstrin homology domains containing protein 3 antibody
    • CENTD3 antibody
    • FLJ21065 antibody
    • Phosphoinositide binding protein antibody
    • PtdIns 3 4 5 P3 binding protein antibody
    • RHO GAP antibody
    see all

图片

  • All lanes : Anti-ARAP3 antibody (ab5911) at 1/500 dilution

    Lane 1 : HEK293 negative control
    Lane 2 : HEK293 overexpressed GFP-ARAP3
    Lane 3 : Whole tissue lysate prepared from mouse heart
    Lane 4 : Whole tissue lysate prepared from mouse brain
    Lane 5 : Whole tissue lysate prepared from mouse diaphragma
    Lane 6 : Whole tissue lysate prepared from mouse fundus
    Lane 7 : Whole tissue lysate prepared from rabbit heart

    Lysates/proteins at 100 µg per lane.

    Secondary
    All lanes : Alexa-Fluor 680 conjugated donkey polyclonal at 1/10000 dilution


    Expected band for HEK overexpression 200kDa.
    Expected band for rabbit heart 170kDa.

    See Abreview

文献

This product has been referenced in:
  • Yagi R  et al. ARAP3 inhibits peritoneal dissemination of scirrhous gastric carcinoma cells by regulating cell adhesion and invasion. Oncogene : (2010). WB, IHC-P, IP ; Human . Read more (PubMed: 21076469) »

See 1 Publication for this product

客户评价及客户问答

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (HEART. BRAIN,DIAPHRAGMA,FUNDUS)
Loading amount
100 µg
Specification
HEART. BRAIN,DIAPHRAGMA,FUNDUS
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Oddysey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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提交于 May 02 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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