Anti-ARAP1抗体[ARAP1-2] (ab64605)

概述

  • 产品名称Anti-ARAP1抗体[ARAP1-2]
    参阅全部 ARAP1 一抗
  • 描述
    小鼠单克隆抗体[ARAP1-2] to ARAP1
  • 特异性This antibody reacts with C-terminal part of ARAP1, a 160 kDa adaptor protein.
  • 经测试应用适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    His6-tagged protein corresponding to amino acids 1190-1450 of human ARAP1.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明> 95% (by SDS-PAGE).
  • 克隆单克隆
  • 克隆编号ARAP1-2
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab64605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 136 kDa.
IP Use at an assay dependent dilution.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Phosphatidylinositol-3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol-3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5.
  • 组织特异性Detected in heart, skeletal muscle, spleen, kidney, liver, placenta, lung, peripheral blood leukocytes, adrenal gland, bone marrow, brain, lymph node, mammary gland, prostate, spinal cord, stomach, thyroid and trachea.
  • 序列相似性Contains 1 Arf-GAP domain.
    Contains 4 PH domains.
    Contains 1 Ras-associating domain.
    Contains 1 Rho-GAP domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • 细胞定位Cytoplasm. Golgi apparatus > Golgi stack membrane. Cell membrane. Associated with Golgi stacks in resting cells. Throughout the cytoplasm and in surface protrusion in cells that are in the process of attaching to a surface and spreading.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2410002L19Rik antibody
    • ANK repeat and PH domain-containing protein 1 antibody
    • ARAP 1 antibody
    • Arap1 antibody
    • ARAP1_HUMAN antibody
    • Arf GAP Rho GAP ankyrin repeat and pleckstrin homology domains containing protein 1 antibody
    • Arf GAP Rho GAP ankyrin repeat and pleckstrin homology domain containing protein 1 antibody
    • Arf-GAP with Rho-GAP domain antibody
    • CENT D2 antibody
    • Centaurin delta 2 antibody
    • Centaurin delta2 antibody
    • Centaurin-delta-2 antibody
    • CENTD 2 antibody
    • CENTD2 antibody
    • Cnt d2 antibody
    • Cnt-d2 antibody
    • Cntd2 antibody
    • KIAA0782 antibody
    • MGC176140 antibody
    • mKIAA0782 antibody
    see all

Anti-ARAP1 antibody [ARAP1-2] 图像

  • ICC/IF image of ab64605 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64605, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-ARAP1 antibody [ARAP1-2] (ab64605)参考文献

ab64605 has not yet been referenced specifically in any publications.

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