Phosphatidylinositol-3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol-3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5.
Detected in heart, skeletal muscle, spleen, kidney, liver, placenta, lung, peripheral blood leukocytes, adrenal gland, bone marrow, brain, lymph node, mammary gland, prostate, spinal cord, stomach, thyroid and trachea.
Cytoplasm. Golgi apparatus > Golgi stack membrane. Cell membrane. Associated with Golgi stacks in resting cells. Throughout the cytoplasm and in surface protrusion in cells that are in the process of attaching to a surface and spreading.
ANK repeat and PH domain-containing protein 1 antibody
ARAP 1 antibody
Arf GAP Rho GAP ankyrin repeat and pleckstrin homology domains containing protein 1 antibody
Arf GAP Rho GAP ankyrin repeat and pleckstrin homology domain containing protein 1 antibody
Arf-GAP with Rho-GAP domain antibody
CENT D2 antibody
Centaurin delta 2 antibody
Centaurin delta2 antibody
CENTD 2 antibody
Cnt d2 antibody
Western blot - ARAP1 antibody (ab99382)
All lanes : Anti-ARAP1 antibody (ab99382) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 136 kDa
Exposure time: 30 seconds
Immunoprecipitation - ARAP1 antibody (ab99382)
Detection of ARAP1 by Western Blot of Immunprecipitate.
ab99382 at 1µg/ml staining ARAP1 in HeLa whole cell lysate immunoprecipitated using ab99382 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.