Anti-Aquaporin 8抗体(ab15124)

概述

  • 产品名称Anti-Aquaporin 8抗体
    参阅全部 Aquaporin 8 一抗
  • 描述
    兔多克隆抗体to Aquaporin 8
  • 特异性The 16 AA peptide was found unique to AQP8 without significant homology to any other AQPs
  • 经测试应用适用于: WB, ELISAmore details
    不适用于: IHC
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    A 16 aa synthetic peptide (Rat) (C terminal). The domain is predicted to be cytoplasmic.

性能

应用

Our Abpromise guarantee covers the use of ab15124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: 1/10000 - 1/50000.
    WB: 1/1000 - 1/5000. Predicted molecular weight: 29 kDa. Use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations is recommended in order to suppress non-specific bands.

    Is unsuitable for IHC.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Forms a water-specific channel; mercury-sensitive. Not permeable to glycerol or urea.
    • 组织特异性Expressed only in pancreas and colon.
    • 序列相似性Belongs to the MIP/aquaporin (TC 1.A.8) family.
    • 结构域Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AQP 8 antibody
      • AQP-8 antibody
      • AQP8 antibody
      • AQP8_HUMAN antibody
      • Aquaporin 8 antibody
      • Aquaporin-8 antibody
      • Aquaporin8 antibody
      see all

    Anti-Aquaporin 8 antibody (ab15124)参考文献

    ab15124 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Pig Tissue lysate - whole (colon)
    Loading amount 60 µg
    Specification colon
    Gel Running Conditions Reduced Denaturing (12% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 21 2011

    Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab15124. Are you using human samples? What dilutions of ab15124 have you tried? At this point I would suggest using it at a 1:1000 dilution and incubate overnigh...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"