重组Anti-Aquaporin 5抗体[EPR3747] (ab92320)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3747] to Aquaporin 5
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Aquaporin 5抗体[EPR3747]
参阅全部 Aquaporin 5 一抗 -
描述
兔单克隆抗体[EPR3747] to Aquaporin 5 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3747 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92320于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
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IHC-P | (3) |
1/1200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended as stronger signals have been found using these. For unpurified use between 1/100 and 1/250.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa. |
IHC-P
1/1200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended as stronger signals have been found using these. For unpurified use between 1/100 and 1/250.
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靶标
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功能
Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Required for TRPV4 activation by hypotonicity (PubMed:16571723). Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed:16571723). -
疾病相关
Keratoderma, palmoplantar, Bothnian type -
序列相似性
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
结构域
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
细胞定位
Apical cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 362 Human
- Omim: 600442 Human
- SwissProt: P55064 Human
- Unigene: 298023 Human
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别名
- AQP 5 antibody
- AQP-5 antibody
- AQP5 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue sections labelling Aquaporin 5 with purified ab92320 at 1/1200 dilution (0.13 µg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
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All lanes : Anti-Aquaporin 5 antibody [EPR3747] (ab92320) at 1/1000 dilution (Purified)
Lane 1 : SW480 (Human colorectal adenocarcinoma epithelial cell) prepared in RIPA lysis method whole cell lysate
Lane 2 : SW480 (Human colorectal adenocarcinoma epithelial cell) prepared in 1%SDS Hot lysis method whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?1%SDS Hot lysis method is recommended for this antibody. The molecular weight observed is consistent with what has been described in the literatures (PMID: 32901888, 23313152).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (45)
ab92320 被引用在 45 文献中.
- Cheng CC et al. Overexpression of N-acetylglucosaminyltransferase V promotes human parotid gland acinar cell immortalization via the epidermal receptor activation. J Cell Physiol 237:1780-1789 (2022). PubMed: 34806177
- Tran E et al. Development of human alveolar epithelial cell models to study distal lung biology and disease. iScience 25:103780 (2022). PubMed: 35169685
- Schmidt H et al. Serially passaged, conditionally reprogrammed nasal epithelial cells as a model to study epithelial functions and SARS-CoV-2 infection. Am J Physiol Cell Physiol 322:C591-C604 (2022). PubMed: 35196166
- Welzel J et al. A novel cell line from human eccrine sweat gland duct cells for investigating sweating physiology. Int J Cosmet Sci 44:216-231 (2022). PubMed: 35262932
- Mauduit O et al. A mesenchymal to epithelial switch in Fgf10 expression specifies an evolutionary-conserved population of ionocytes in salivary glands. Cell Rep 39:110663 (2022). PubMed: 35417692