概述

  • 产品名称
    Anti-Aquaporin 5抗体
    参阅全部 Aquaporin 5 一抗
  • 描述
    兔多克隆抗体to Aquaporin 5
  • 经测试应用
    适用于: IHC-P, ELISA, WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog
  • 免疫原

    A 17 amino acid synthetic peptide corresponding to the carboxy terminus of rat Aquaporin 5

  • 阳性对照
    • Rat salivary gland lysate.

性能

应用

Our Abpromise guarantee covers the use of ab78486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 2 - 10 µg/ml.
ELISA Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.05 - 0.1 µg/ml. Predicted molecular weight: 29 kDa.
IHC-Fr Use at an assay dependent concentration. PubMed: 21704616

靶标

  • 功能
    Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Required for TRPV4 activation by hypotonicity (PubMed:16571723). Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed:16571723).
  • 疾病相关
    Keratoderma, palmoplantar, Bothnian type
  • 序列相似性
    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • 结构域
    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • 细胞定位
    Apical cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AQP 5 antibody
    • AQP-5 antibody
    • AQP5 antibody
    • AQP5_HUMAN antibody
    • Aquaporin 5 antibody
    • Aquaporin-5 antibody
    see all

图片

  • Lane 1 : Anti-Aquaporin 5 antibody (ab78486) at 0.05 µg/ml
    Lane 2 : Anti-Aquaporin 5 antibody (ab78486) at 0.1 µg/ml

    Lane 1 : rat submaxillary (salivary) gland lysate
    Lane 2 : rat submaxillary (salivary) gland lysate


    Predicted band size : 29 kDa
    Observed band size : 29 kDa

文献

This product has been referenced in:
  • Tong L  et al. Fibroblast Growth Factor-10 (FGF-10) Mobilizes Lung-resident Mesenchymal Stem Cells and Protects Against Acute Lung Injury. Sci Rep 6:21642 (2016). IF ; Rat . Read more (PubMed: 26869337) »
  • Cao Z  et al. Targeting of the pulmonary capillary vascular niche promotes lung alveolar repair and ameliorates fibrosis. Nat Med 22:154-62 (2016). Read more (PubMed: 26779814) »

See all 16 Publications for this product

客户评价及客户问答

Application
CyTOF
Sample
Mouse Cell (Lung)
Specification
Lung
Username

Irit Milman

Verified customer

提交于 Nov 14 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Lung)
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris NuPAGE)
Loading amount
40 µg
Specification
Lung
Blocking step
Odyssey TBS Blocking Buffer (proprietary) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Username

Pete Serbedzija

Verified customer

提交于 May 25 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (HEK cells transfected with AQP5-EGFP)
Permeabilization
Yes - 0,2% Saponin
Specification
HEK cells transfected with AQP5-EGFP
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 03 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (primarly lung epithelia cells)
Specification
primarly lung epithelia cells
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 0.2% · Temperature: 21°C
Fixative
Methanol
Username

Abcam user community

Verified customer

提交于 Oct 08 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Enzymatic
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 20 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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