The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 - 10 µg/ml.
Use a concentration of 0.5 - 1 µg/ml.
Use a concentration of 0.05 - 0.1 µg/ml. Predicted molecular weight: 29 kDa.
Use at an assay dependent concentration. PubMed: 21704616
功能Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Required for TRPV4 activation by hypotonicity (PubMed:16571723). Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed:16571723).
疾病相关Keratoderma, palmoplantar, Bothnian type
序列相似性Belongs to the MIP/aquaporin (TC 1.A.8) family.
结构域Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Lane 1 : Anti-Aquaporin 5 antibody (ab78486) at 0.05 µg/ml Lane 2 : Anti-Aquaporin 5 antibody (ab78486) at 0.1 µg/ml
Lane 1 : rat submaxillary (salivary) gland lysate Lane 2 : rat submaxillary (salivary) gland lysate
Predicted band size : 29 kDa Observed band size : 29 kDa
Anti-Aquaporin 5 antibody (ab78486)参考文献
This product has been referenced in:
Whiteman EL et al. Crumbs3 is essential for proper epithelial development and viability. Mol Cell Biol34:43-56 (2014).
Read more (PubMed: 24164893) »
Hai B et al. Transient activation of hedgehog pathway rescued irradiation-induced hyposalivation by preserving salivary stem/progenitor cells and parasympathetic innervation. Clin Cancer Res20:140-50 (2014).
Read more (PubMed: 24150232) »