概述

  • 产品名称Anti-Aquaporin 5抗体
    参阅全部 Aquaporin 5 一抗
  • 描述
    兔多克隆抗体to Aquaporin 5
  • 经测试应用适用于: IHC-P, ELISA, WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog
  • 免疫原

    A 17 amino acid synthetic peptide corresponding to the carboxy terminus of rat Aquaporin 5

  • 阳性对照
    • Rat salivary gland lysate.

性能

应用

Our Abpromise guarantee covers the use of ab78486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 2 - 10 µg/ml.
ELISA Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.05 - 0.1 µg/ml. Predicted molecular weight: 29 kDa.
IHC-Fr Use at an assay dependent concentration. PubMed: 21704616

靶标

  • 功能Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Required for TRPV4 activation by hypotonicity (PubMed:16571723). Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed:16571723).
  • 疾病相关Keratoderma, palmoplantar, Bothnian type
  • 序列相似性Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • 结构域Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • 细胞定位Apical cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AQP 5 antibody
    • AQP-5 antibody
    • AQP5 antibody
    • AQP5_HUMAN antibody
    • Aquaporin 5 antibody
    • Aquaporin-5 antibody
    see all

Anti-Aquaporin 5 antibody 图像

  • Lane 1 : Anti-Aquaporin 5 antibody (ab78486) at 0.05 µg/ml
    Lane 2 : Anti-Aquaporin 5 antibody (ab78486) at 0.1 µg/ml

    Lane 1 : rat submaxillary (salivary) gland lysate
    Lane 2 : rat submaxillary (salivary) gland lysate


    Predicted band size : 29 kDa
    Observed band size : 29 kDa

Anti-Aquaporin 5 antibody (ab78486)参考文献

This product has been referenced in:
  • Lin SE  et al. Expression of human carcinoembryonic antigen-related cell adhesion molecule 6 and alveolar progenitor cells in normal and injured lungs of transgenic mice. Physiol Rep 3: (2015). IHC-Fr . Read more (PubMed: 26702074) »
  • Whiteman EL  et al. Crumbs3 is essential for proper epithelial development and viability. Mol Cell Biol 34:43-56 (2014). WB ; Mouse . Read more (PubMed: 24164893) »

See all 10 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Lung)
Gel Running Conditions Reduced Denaturing (12% Bis-Tris NuPAGE)
Loading amount 40 µg
Specification Lung
Blocking step Odyssey TBS Blocking Buffer (proprietary) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Username

Pete Serbedzija

Verified customer

提交于 May 25 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (HEK cells transfected with AQP5-EGFP)
Permeabilization Yes - 0,2% Saponin
Specification HEK cells transfected with AQP5-EGFP
Blocking step Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 03 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (primarly lung epithelia cells)
Specification primarly lung epithelia cells
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 0.2% · Temperature: 21°C
Fixative Methanol
Username

Abcam user community

Verified customer

提交于 Oct 08 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Enzymatic
Sample Mouse Tissue sections (Lung)
Specification Lung
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 20 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"