Anti-Aquaporin 4抗体[4/18] (ab9512)

概述

  • 产品名称Anti-Aquaporin 4抗体[4/18]
    参阅全部 Aquaporin 4 一抗
  • 描述
    小鼠单克隆抗体[4/18] to Aquaporin 4
  • 经测试应用适用于: ELISA, IHC-Fr, IHC-P, WB, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human, Zebrafish
    预测可用于: Cow
  • 免疫原

    Synthetic peptide:

    VIDIDRGDEKKGKDSSGE

    , corresponding to amino acids 301-318 of Aquaporin 4

  • 表位Intracellular C-terminal AQP 4 epitope

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, 1mg/ml BSA
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆单克隆
  • 克隆编号4/18
  • 同种型IgG3
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration. PubMed: 11595449

Also Pubmed ID: 12172575

IHC-Fr Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. PubMed: 19831719
IHC-FoFr Use at an assay dependent concentration. PubMed: 22438975

靶标

  • 功能Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
  • 组织特异性Brain - muscle >> heart, kidney, lung, and trachea.
  • 序列相似性Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • 结构域Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • 翻译后修饰Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40%.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AQP 4 antibody
    • AQP-4 antibody
    • AQP4 antibody
    • AQP4_HUMAN antibody
    • Aquaporin type 4 antibody
    • Aquaporin-4 antibody
    • Aquaporin4 antibody
    • HMIWC 2 antibody
    • HMIWC2 antibody
    • Mercurial insensitive water channel antibody
    • Mercurial-insensitive water channel antibody
    • MGC22454 antibody
    • MIWC antibody
    • WCH 4 antibody
    • WCH4 antibody
    see all

Anti-Aquaporin 4 antibody [4/18] 图像

  • Immunohistochemical staining of aquaporin 4 in rat brain.
  • Immunohistochemical analysis of mouse brain tissue, staining Aquaporin 4 with ab9512 at 1/100 dilution. Staining was detected using a biotin-conjugated anti-mouse antibody followed by FITC-conjugated streptavidin.

Anti-Aquaporin 4 antibody [4/18] (ab9512)参考文献

This product has been referenced in:
  • Leonard AV  et al. NK1 receptor blockade is ineffective in improving outcome following a balloon compression model of spinal cord injury. PLoS One 9:e98364 (2014). IHC-P ; Rabbit . Read more (PubMed: 24859234) »
  • Glover LE  et al. Immediate, but not delayed, microsurgical skull reconstruction exacerbates brain damage in experimental traumatic brain injury model. PLoS One 7:e33646 (2012). IHC-FoFr ; Rat . Read more (PubMed: 22438975) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Ph9 Microwave
Permeabilization No
Specification Brain
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1500µg/mL · Temperature: RT°C
Fixative Paraformaldehyde
Username

Gaia Brezzo

Verified customer

提交于 Sep 06 2016

Thank you for this information. It is unusual for one to see a band and then subsequently see nothing, which is why I thought you may have been stripping and re-probing. You may improve your result by increasing the concentration of the primary antibod...

Read More

I am sorry to hear that this antibody is not providing satisfactory results.
You indicated that the secondary antibody used is a rabbit antibody that reacts with rat, however the primary is in mouse so you would need an anti-mouse antibody. Please ...

Read More

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for...

Read More

Thank you for contacting us.

We realize that our products can be considered to have premium price point. However, we feel that the product and service which we offer are of the highest possible quality. Under our Abpromise guarantee, ourprod...

Read More

Thank you for contacting us. I am glad to hear that this replacement product is performing better than the last product.

Many factors can alter the predicted molecular weight of a protein. These may include post-translational modifications, ...

Read More

Thank you for your enquiry.

I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any Aquaporin 4 antibody tested in th...

Read More

I have asked that an FOCR be created for ab9512 of a different lot number. You should be receiving confirmation shortly and it should be included with your next order. Please let me know if you have any questions.

Thank you for contacting us. I would like to your thank customer for their extensive troubleshooting efforts and time. I really do not understand why this particular product has given your customer problem. This product should have worked in their ass...

Read More

Thank you for contacting Abcam. As the product has been used in a way that is consistent with our Abpromise guarantee we can replace it with either a new vial or lot of the same product or with a new antibody against Aquaporin 4. We offer 5 others all ...

Read More

1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"