Anti-Aquaporin 4抗体[3D2] (ab11026)

概述

  • 产品名称
    Anti-Aquaporin 4抗体[3D2]
    参阅全部 Aquaporin 4 一抗
  • 描述
    小鼠单克隆抗体[3D2] to Aquaporin 4
  • 特异性
    ab11026 recognises an epitope within the cytoplasmic domain of the water-specific channel aquaporin 4.
  • 经测试应用
    适用于: IHC-FoFr, WB, IHC-P, IHC-Fr, IP, ELISAmore details
  • 种属反应性
    与反应: Human, Pig
    不与反应: Mouse
  • 免疫原

    Synthetic peptide, corresponding to amino acids 301-318 of Rat Aquaporin 4.

  • 阳性对照
    • Kidney tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    Purified from tissue culture supernatant.
  • 克隆
    单克隆
  • 克隆编号
    3D2
  • 骨髓瘤
    Sp2/0-Ag14
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab11026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 17398107
WB 1/1000 - 1/10000. Predicted molecular weight: 38.8 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/100.
IP 1/1000 - 1/10000.
ELISA 1/1000 - 1/20000.

靶标

  • 功能
    Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
  • 组织特异性
    Brain - muscle >> heart, kidney, lung, and trachea.
  • 序列相似性
    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • 结构域
    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • 翻译后修饰
    Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40%.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AQP 4 antibody
    • AQP-4 antibody
    • AQP4 antibody
    • AQP4_HUMAN antibody
    • Aquaporin type 4 antibody
    • Aquaporin-4 antibody
    • Aquaporin4 antibody
    • HMIWC 2 antibody
    • HMIWC2 antibody
    • Mercurial insensitive water channel antibody
    • Mercurial-insensitive water channel antibody
    • MGC22454 antibody
    • MIWC antibody
    • WCH 4 antibody
    • WCH4 antibody
    see all

Anti-Aquaporin 4 antibody [3D2] (ab11026)参考文献

This product has been referenced in:
  • Krencik R  et al. Dysregulation of astrocyte extracellular signaling in Costello syndrome. Sci Transl Med 7:286ra66 (2015). ICC . Read more (PubMed: 25947161) »
  • Parfenova H  et al. Functional role of astrocyte glutamate receptors and carbon monoxide in cerebral vasodilation response to glutamate. Am J Physiol Heart Circ Physiol 302:H2257-66 (2012). ICC/IF ; Pig . Read more (PubMed: 22467311) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Monkey Tissue sections (retina)
Specification
retina
Username

Abcam user community

Verified customer

提交于 Jul 25 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (CHO cells stably expressing human AQP4. Control c)
Gel Running Conditions
Reduced Denaturing (12% BioRad Gel)
Loading amount
20 µg
Specification
CHO cells stably expressing human AQP4. Control c
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Ramon Wade

Verified customer

提交于 May 22 2017

The choice of secondary antibody will depend on the application you are using the antibody for. For western blotting with electrochemiluminescence (ECL) detection, we recommend an anti-mouse IgG secondary antibody conjugated to horseradish peroxidase (...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Brain)
Specification
Human Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 15µg/mL · Temperature: 21°C
Username

Dr. Lucy Craggs

Verified customer

提交于 Oct 04 2012

DISCOUNT CODE: XXXXXXXXXX
Expiration date: [12/11/2012]
I am very pleased to hear you would like to use ab11026 in IHC-P and send us an image of your results.
This code will give you 1 free [PRIMARY ANTIBODY/PROTEIN] before the expiration...

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Thank you for your inquiry.


A suitable secondary antibody for ab11026 would be ab6728Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP).


http://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Mouse-I...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain)
Specification
brain
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5%
Username

Vlady Pavlova

Verified customer

提交于 Oct 05 2006

Thank you for getting back in touch with me and for clarifying this issue. I considered this a possibility given that you are using a HRP detection system. Once again I am disapointed to hear that you have been obtained cytoplasm staining with this ...

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Thank you for your enquiry. Once again I am sorry to hear that you have not managed to obtain the expected staining pattern of this antibody. I have read your technical questionnaire and you have tried many of the optimisation steps that I would re...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. The only details I have is that Aquaporin 4 is a water specific plasma membrane channel protein and that ab11026 recognizes an epitope within ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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