Anti-Aquaporin 2 (phospho S256)抗体(ab111346)

概述

  • 产品名称
    Anti-Aquaporin 2 (phospho S256)抗体
    参阅全部 Aquaporin 2 一抗
  • 描述
    兔多克隆抗体to Aquaporin 2 (phospho S256)
  • 宿主
    Rabbit
  • 特异性
    ab111346 detects endogenous levels of Aquaporin 2 only when phosphorylated at serine 256.
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide phosphopeptide derived from Human Aquaporin 2 around the phosphorylation site of serine 256 (R-Q-SP-V-E).

  • 阳性对照
    • Human brain tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS (without Mg2+ and Ca2+)
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab111346 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab111346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • 组织特异性
    Expressed in renal collecting tubules.
  • 疾病相关
    Diabetes insipidus, nephrogenic, autosomal
  • 序列相似性
    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • 结构域
    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • 翻译后修饰
    Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
  • 细胞定位
    Apical cell membrane. Basolateral cell membrane. Cytoplasmic vesicle membrane. Golgi apparatus, trans-Golgi network membrane. Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADH water channel antibody
    • AQP 2 antibody
    • AQP CD antibody
    • AQP-2 antibody
    • AQP-CD antibody
    • AQP2 antibody
    • AQP2_HUMAN antibody
    • AQPCD antibody
    • Aquaporin 2 collecting duct antibody
    • Aquaporin CD antibody
    • Aquaporin-2 antibody
    • Aquaporin-CD antibody
    • Aquaporin2 antibody
    • Aquaporine 2 antibody
    • Collecting duct water channel protein antibody
    • MGC34501 antibody
    • Water channel aquaporin 2 antibody
    • Water channel protein for renal collecting duct antibody
    • WCH CD antibody
    • WCH-CD antibody
    • WCHCD antibody
    see all

图片

  • ab111346 at 1/50 dilution staining Aquaporin 2 in Paraffin-embedded Human Brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

文献

ab111346 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Mouse Cell lysate - whole cell (IMCD Cells)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
25 µg
Treatment
- and + 500 ´M dbcAMP
Specification
IMCD Cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mrs. Andrea Geelhaar

Verified customer

提交于 May 30 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (IMCD Cells)
Gel Running Conditions
Reduced Denaturing (12 %)
Loading amount
25 µg
Treatment
- and + 100 nM AVP
Specification
IMCD Cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mrs. Andrea Geelhaar

Verified customer

提交于 Feb 22 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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