Anti-Aquaporin 2 (phospho S256)抗体(ab111346)
Key features and details
- Rabbit polyclonal to Aquaporin 2 (phospho S256)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-Aquaporin 2 (phospho S256)抗体
参阅全部 Aquaporin 2 一抗 -
描述
兔多克隆抗体to Aquaporin 2 (phospho S256) -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Aquaporin 2 (phospho S256).
-
阳性对照
- Human brain tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS (without Mg2+ and Ca2+) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
ab111346 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab111346于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
---|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. -
组织特异性
Expressed in renal collecting tubules. -
疾病相关
Diabetes insipidus, nephrogenic, autosomal -
序列相似性
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
结构域
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
翻译后修饰
Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent. -
细胞定位
Apical cell membrane. Basolateral cell membrane. Cytoplasmic vesicle membrane. Golgi apparatus, trans-Golgi network membrane. Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated. - Information by UniProt
-
数据库链接
- Entrez Gene: 359 Human
- Entrez Gene: 11827 Mouse
- Entrez Gene: 25386 Rat
- Omim: 107777 Human
- SwissProt: P41181 Human
- SwissProt: P56402 Mouse
- SwissProt: P34080 Rat
- Unigene: 130730 Human
see all -
别名
- ADH water channel antibody
- AQP 2 antibody
- AQP CD antibody
see all
图片
数据表及文件
-
SDS download
-
Datasheet download
文献 (4)
ab111346 被引用在 4 文献中.
- Kim S et al. Psychotropic drugs upregulate aquaporin-2 via vasopressin-2 receptor/cAMP/protein kinase A signaling in inner medullary collecting duct cells. Am J Physiol Renal Physiol 320:F963-F971 (2021). PubMed: 33843270
- Wang R et al. Pdcd10-Stk24/25 complex controls kidney water reabsorption by regulating Aqp2 membrane targeting. JCI Insight 6:N/A (2021). PubMed: 34156031
- Kong Y et al. Statins ameliorate cholesterol-induced inflammation and improve AQP2 expression by inhibiting NLRP3 activation in the kidney. Theranostics 10:10415-10433 (2020). PubMed: 32929357
- Huang J et al. Arginase-II negatively regulates renal aquaporin-2 and water reabsorption. FASEB J 32:5520-5531 (2018). PubMed: 29718707