Anti-Aquaporin 2抗体(ab15116)

概述

  • 产品名称
    Anti-Aquaporin 2抗体
    参阅全部 Aquaporin 2 一抗
  • 描述
    兔多克隆抗体to Aquaporin 2
  • 经测试应用
    适用于: IHC-P, ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Human, Pig
  • 免疫原

    A 15 aa synthetic MAP peptide (Rat) (C terminal). This peptide is predicted to be cytoplasmic.

性能

应用

Our Abpromise guarantee covers the use of ab15116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 21576461
ICC/IF 1/500.
WB 1/1000 - 1/5000. Predicted molecular weight: 29 kDa.
ELISA 1/10000 - 1/50000.

靶标

  • 功能
    Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • 组织特异性
    Expressed in renal collecting tubules.
  • 疾病相关
    Diabetes insipidus, nephrogenic, autosomal
  • 序列相似性
    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • 结构域
    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • 翻译后修饰
    Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
  • 细胞定位
    Apical cell membrane. Basolateral cell membrane. Cytoplasmic vesicle membrane. Golgi apparatus, trans-Golgi network membrane. Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADH water channel antibody
    • AQP 2 antibody
    • AQP CD antibody
    • AQP-2 antibody
    • AQP-CD antibody
    • AQP2 antibody
    • AQP2_HUMAN antibody
    • AQPCD antibody
    • Aquaporin 2 collecting duct antibody
    • Aquaporin CD antibody
    • Aquaporin-2 antibody
    • Aquaporin-CD antibody
    • Aquaporin2 antibody
    • Aquaporine 2 antibody
    • Collecting duct water channel protein antibody
    • MGC34501 antibody
    • Water channel aquaporin 2 antibody
    • Water channel protein for renal collecting duct antibody
    • WCH CD antibody
    • WCH-CD antibody
    • WCHCD antibody
    see all

文献

This product has been referenced in:
  • Whiting JL  et al. AKAP220 manages apical actin networks that coordinate aquaporin-2 location and renal water reabsorption. Proc Natl Acad Sci U S A 113:E4328-37 (2016). WB . Read more (PubMed: 27402760) »
  • Dirocco DP  et al. Wnt4/ß-Catenin Signaling in Medullary Kidney Myofibroblasts. J Am Soc Nephrol 24:1399-412 (2013). Read more (PubMed: 23766539) »

See all 6 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 25°C
Sample
Mouse Cell (mouse kidney TISSUE)
Specification
mouse kidney TISSUE
Permeabilization
Yes - triton-X
Fixative
Acetone
Username

Abcam user community

Verified customer

提交于 Aug 08 2013

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The Immunogen for ab9566Anti-Aquaporin 1 ...

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