Anti-Aquaporin 2抗体(ab110496)

概述

  • 产品名称
    Anti-Aquaporin 2抗体
    参阅全部 Aquaporin 2 一抗
  • 描述
    兔多克隆抗体to Aquaporin 2
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Sheep, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Aquaporin 2.

  • 阳性对照
    • This antibody gave a positive signal in Human, Mouse and Rat Kidney tissue lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab110496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 29 kDa).

靶标

  • 功能
    Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • 组织特异性
    Expressed in renal collecting tubules.
  • 疾病相关
    Diabetes insipidus, nephrogenic, autosomal
  • 序列相似性
    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • 结构域
    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • 翻译后修饰
    Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
  • 细胞定位
    Apical cell membrane. Basolateral cell membrane. Cytoplasmic vesicle membrane. Golgi apparatus, trans-Golgi network membrane. Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADH water channel antibody
    • AQP 2 antibody
    • AQP CD antibody
    • AQP-2 antibody
    • AQP-CD antibody
    • AQP2 antibody
    • AQP2_HUMAN antibody
    • AQPCD antibody
    • Aquaporin 2 collecting duct antibody
    • Aquaporin CD antibody
    • Aquaporin-2 antibody
    • Aquaporin-CD antibody
    • Aquaporin2 antibody
    • Aquaporine 2 antibody
    • Collecting duct water channel protein antibody
    • MGC34501 antibody
    • Water channel aquaporin 2 antibody
    • Water channel protein for renal collecting duct antibody
    • WCH CD antibody
    • WCH-CD antibody
    • WCHCD antibody
    see all

图片

  • All lanes : Anti-Aquaporin 2 antibody (ab110496) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Kidney (Mouse) Tissue Lysate
    Lane 3 : Kidney (Rat) Tissue Lysate
    Lane 4 : Human kidney tissue lysate - total protein (ab30203) with Immunising peptide at 1 µg/ml
    Lane 5 : Kidney (Mouse) Tissue Lysate with Immunising peptide at 1 µg/ml
    Lane 6 : Kidney (Rat) Tissue Lysate with Immunising peptide at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 22 kDa, 53 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 16 minutes


    Aquaporin 2 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.

实验方案

文献

ab110496 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate buffer
Permeabilization
No
Specification
Kidney
Blocking step
Fish Gelatin in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 21°C
Fixative
Paraformaldehyde
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提交于 Nov 20 2017

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