概述

  • 产品名称Anti-Aquaporin 1抗体
    参阅全部 Aquaporin 1 一抗
  • 描述
    兔多克隆抗体to Aquaporin 1
  • 经测试应用适用于: ICC/IF, IHC-P, WB, ELISA, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Cow, Dog, Human, Pig
  • 免疫原

    A 19 amino acid synthetic peptide within the carboxy terminal domain of rat Aquaporin 1 that is 100% homologous in mouse, rat, human and Bovine. This domain is predicted to be cytoplasmic.

  • 阳性对照
    • rat kidney lysate, rat heart lysate

性能

应用

Our Abpromise guarantee covers the use of ab15080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-P 1/500.
WB 1/1000 - 1/5000. Predicted molecular weight: 32 kDa.Can be blocked with Aquaporin 1 peptide (ab25381).
ELISA 1/10000 - 1/50000.
IHC-Fr 1/500.

靶标

  • 功能Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • 组织特异性Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
  • 序列相似性Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • 结构域Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AQP 1 antibody
    • AQP CHIP antibody
    • AQP-1 antibody
    • AQP1 antibody
    • AQP1_HUMAN antibody
    • aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) antibody
    • aquaporin 1 (Colton blood group) antibody
    • Aquaporin CHIP antibody
    • Aquaporin-1 antibody
    • Aquaporin-CHIP antibody
    • Aquaporin1 antibody
    • Channel forming integral protein 28kDa antibody
    • Channel like integral membrane protein 28 kDa antibody
    • CHIP 28 antibody
    • CHIP28 antibody
    • CO antibody
    • Colton blood group antibody
    • Growth factor induced delayed early response protein antibody
    • MGC26324 antibody
    • Urine water channel antibody
    • Water channel protein CHIP 29 antibody
    • Water channel protein CHIP29 antibody
    • Water channel protein for red blood cells and kidney proximal tubule antibody
    see all

Anti-Aquaporin 1 antibody 图像

  • ab15080 staining Aquaporin 1 in human kidney tubule tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde. Antigen retrieval was by heat mediation in citrate buffer (20 minutes at 100°C). Samples were incubated with primary antibody 1/2000(background reducer diluent) for 20 minutes at 25°C. An undiluted HRP polymer Goat anti mouse/rabbit monoclonal IgG was used as secondary antibody.

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  • ab15080 staining Aquaporin 1 in the intervertebral disc (nucleus pulposus) of the cow cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 10% serum for 30 minutes at 21°C. Samples were incubated with primary antibody (1/200) for 24 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/200).

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  • ab15080 staining Aquaporin1 in porcine jejunum by immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde without undergoing permeabilization and blocking steps. The primary antibody diluted 1/500 and incubated with sample for 24 hours. A Texas Red® conjugated goat polyclonal IgG whole molecule to rabbit, diluted 1/400 was used as the secondary.

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  • ab15080 staining Aquaporin 1 in rat 9L (gliosarcoma) cells by Immunocytochemistry.

    Cells were kept under normoxic conditions (upper panel) or exposed to hypoxia (lower panel). Cells were then fixed with paraformaldehyde, permeabilized in 0.1% Triton X-100 in PBS, before being blocked with 10% fetal calf serum + 1 mg/ml BSA in PBS overnight at 4°C. Samples were subsequently incubated with primary antibody (1/500 in diluent) and a peroxidase-conjugated goat anti-rabbit IgG was used as a secondary antibody. Samples were stained using DAB and counter-stained with hematoxylin.
  • ab15080 at 1/500 dilution, staining Aquaporin 1 in sheep duodenum section by immunohistochemistry (Frozen sections). Sections were paraformaldehyde fixed and incubated with ab15080, for 24 hours. A Texas Red® conjugated goat polyclonal to rabbit Ig, diluted 1/400 was used as the secondary antibody.

    See Abreview

Anti-Aquaporin 1 antibody (ab15080)参考文献

This product has been referenced in:
  • Méndez-Gómez HR  et al. Transcytosis in the blood-cerebrospinal fluid barrier of the mouse brain with an engineered receptor/ligand system. Mol Ther Methods Clin Dev 2:15037 (2015). IHC-FrFl ; Mouse . Read more (PubMed: 26491705) »
  • Calero C  et al. Expression of aquaporins in bronchial tissue and lung parenchyma of patients with chronic obstructive pulmonary disease. Multidiscip Respir Med 9:29 (2014). IHC-P ; Human . Read more (PubMed: 24917931) »

See all 13 Publications for this product

Product Wall

Application IHC - Wholemount
Sample Human Tissue (Lens Capsule)
Specification Lens Capsule
Username

Mr. Manuel Chacon

Verified customer

提交于 Aug 25 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
Sample Cow Cell (Intervertebral disc (nucleus pulposus))
Specification Intervertebral disc (nucleus pulposus)
Permeabilization Yes - 0.5% Triton-X 100
Fixative Paraformaldehyde
Username

Rob

Verified customer

提交于 Jul 03 2013

I have been able to confirm that this product is provided in 40% glycerol. For longer term storage we recommend bringing the total glycerol content up to 50% and stroage at -20C. In order to do this, please at 5ul of glycerol to the vial and mix.

Thank you for contacting us.

Please put the antibody in refrigerator at 4C over the weekend.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our ...

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Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on th...

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Gracias por contactarnos.

Lamento mucho las molestias ocasionadas por la falta de volumen en el vial de ab15080 recibido.

¿Habéis centrifugado el vial y comprobado que el líquido no haya quedado adherido a las p...

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Thank you for your inquiry. The Anti-Aquaporin 1 antibody ab15080 is provided as whole antiserum. Such unpurified antibodies will not have a concentration stated on the datasheet, as for most whole antiserum, culture supernatant or ascites fluid pr...

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Thank you for contacting Abcam about ab15080.   The immunogen is the C-terminal region of Aquaporin 1.  The immunizing peptide is 100% homologous in Mouse, Rat, Human, and Bovine AQP1.  Because Bovine and Porcine AQP1 have very high homology in this re...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Kidney)
Specification Kidney
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jan 12 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (kideny, tubules)
Specification kideny, tubules
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate, 100C, 20 min
Permeabilization No
Username

Abcam user community

Verified customer

提交于 Nov 20 2009

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"