Anti-Aquaporin 1抗体[7D11] (ab11025)

概述

  • 产品名称
    Anti-Aquaporin 1抗体[7D11]
    参阅全部 Aquaporin 1 一抗
  • 描述
    小鼠单克隆抗体[7D11] to Aquaporin 1
  • 特异性
    ab11025 recognises an epitope within an extracellular region of the water-specific channel aquaporin 1.
  • 经测试应用
    适用于: IHC-P, IHC-Fr, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 198-208 of Rat Aquaporin 1.

  • 阳性对照
    • Kidney tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    Purified from tissue culture supernatant.
  • 克隆
    单克隆
  • 克隆编号
    7D11
  • 骨髓瘤
    Sp2/0-Ag14
  • 同种型
    IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab11025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/500 - 1/1000.
WB 1/1000 - 1/2000. Predicted molecular weight: 32.28 kDa.
ELISA 1/1000 - 1/10000.

靶标

  • 功能
    Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • 组织特异性
    Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
  • 序列相似性
    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • 结构域
    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AQP 1 antibody
    • AQP CHIP antibody
    • AQP-1 antibody
    • AQP1 antibody
    • AQP1_HUMAN antibody
    • aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) antibody
    • aquaporin 1 (Colton blood group) antibody
    • Aquaporin CHIP antibody
    • Aquaporin-1 antibody
    • Aquaporin-CHIP antibody
    • Aquaporin1 antibody
    • Channel forming integral protein 28kDa antibody
    • Channel like integral membrane protein 28 kDa antibody
    • CHIP 28 antibody
    • CHIP28 antibody
    • CO antibody
    • Colton blood group antibody
    • Growth factor induced delayed early response protein antibody
    • MGC26324 antibody
    • Urine water channel antibody
    • Water channel protein CHIP 29 antibody
    • Water channel protein CHIP29 antibody
    • Water channel protein for red blood cells and kidney proximal tubule antibody
    see all

文献

This product has been referenced in:
  • George SK  et al. Potential Use of Autologous Renal Cells from Diseased Kidneys for the Treatment of Renal Failure. PLoS One 11:e0164997 (2016). Read more (PubMed: 27776163) »
  • Kang HM  et al. Sox9-Positive Progenitor Cells Play a Key Role in Renal Tubule Epithelial Regeneration in Mice. Cell Rep 14:861-871 (2016). Read more (PubMed: 26776520) »

See all 4 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain)
Specification
brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5%
Username

Vlady Pavlova

Verified customer

提交于 Aug 16 2006

I'm sorry to hear you are experiencing problems with ab11025. This antibody has not been tested in immunofluorescence and this may be the reason for the problem. I would recommend trying a biotin-ABC-DAB detection system which is more sensitive that ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册