概述

  • 产品名称Anti-APRT抗体
    参阅全部 APRT 一抗
  • 描述
    兔多克隆抗体to APRT
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide selected from the C-terminal region of Human APRT conjugated to KLH (NP_000476).

  • 阳性对照
    • 293 cell lysates transiently transfected with the APRT gene.

性能

应用

Our Abpromise guarantee covers the use of ab91428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 20 kDa.
ELISA 1/1000.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
  • 通路Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.
  • 疾病相关Defects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD) [MIM:102600]; also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones.
  • 序列相似性Belongs to the purine/pyrimidine phosphoribosyltransferase family.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adenine phosphoribosyltransferase antibody
    • AMP antibody
    • AMP diphosphorylase antibody
    • AMP pyrophosphorylase antibody
    • APRT antibody
    • APT_HUMAN antibody
    • DKFZp686D13177 antibody
    • MGC125856 antibody
    • MGC125857 antibody
    • MGC129961 antibody
    • Transphosphoribosidase antibody
    see all

Anti-APRT antibody 图像

  • All lanes : Anti-APRT antibody (ab91428) at 1/100 dilution

    Lane 1 : 293 cell lysates (nontransfected)
    Lane 2 : 293 cell lysates transiently transfected with the APRT gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 20 kDa
  • ICC/IF image of ab91428 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91428, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-APRT antibody (ab91428)参考文献

ab91428 has not yet been referenced specifically in any publications.

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