概述

  • 产品名称Anti-APRIL抗体[Aprily-1]
    参阅全部 APRIL 一抗
  • 描述
    小鼠单克隆抗体[Aprily-1] to APRIL
  • 经测试应用适用于: IP, Flow Cyt, WBmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 免疫原

    Recombinant fragment, corresponding to amino acids 93 - 233 of Human APRIL, expressed in bacteria.

性能

应用

Our Abpromise guarantee covers the use of ab16088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
Flow Cyt
WB
  • 应用说明Flow Cyt: Use at a concentration of 10 µg/ml. This antibody also works for intracellular FC staining.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa.
    IP: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
    • 组织特异性Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages.
    • 序列相似性Belongs to the tumor necrosis factor family.
    • 翻译后修饰The precursor is cleaved by furin.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • A proliferation inducing ligand antibody
      • A proliferation-inducing ligand antibody
      • APRIL antibody
      • CD256 antibody
      • TALL-2 antibody
      • TALL2 antibody
      • TNF related death ligand antibody
      • TNF- and APOL-related leukocyte expressed ligand 2 antibody
      • TNF-related death ligand 1 antibody
      • TNF13_HUMAN antibody
      • TNFSF13 antibody
      • TRDL-1 antibody
      • TRDL1 antibody
      • Tumor necrosis factor (ligand) superfamily, member 13 antibody
      • Tumor necrosis factor ligand superfamily member 13 antibody
      • Tumor necrosis factor like protein ZTNF2 antibody
      • Tumor necrosis factor related death ligand 1 antibody
      • UNQ383/PRO715 antibody
      • ZTNF2 antibody
      see all

    Anti-APRIL antibody [Aprily-1] 图像

    • ab16088 at 10ug/ml detecting APRIL by FACS analysis.

      Left hand panel mock transfected cells

      Right hand panel human APRIL tranfected cells

      HEK 293 cells were mock transfected or transfected with an expression plasmid enabling surface expression of a non-cleavable human APRIL. Cells (5x105) were incubated on ice for 30 min in 50 ul FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 10ug/ml of Aprily-1 antibody. After washing in FACS buffer, FITC-conjugated antibody to mouse IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.



    • Predicted band size : 30 kDa


      Western blot of total cell extracts from HEK 293 cells transfected with the indicated expression vector. Aprily-1 reacts specifically with human APRIL.

      10ug of protein was applied to the gel. Revealed with ab16088 (1ug/ml) and HRP-coupled anti-mouse secondary antibody (1:4000).

      The human APRIL construct used in this experiment is an uncleavable fusion protein between human BAFF (aa 1-132) and human APRIL (aa 93-233).

    Anti-APRIL antibody [Aprily-1] (ab16088)参考文献

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    The immunogen of ab16088 is a recombinant fragment corresponding to amino acids 93 - 233 of Human APRIL.

    The uncleaved protein is 250 amino acids long as is cleaved at amino acid 104-105 into 2 fragments: the propeptide (aa 1-104) and the chai...

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    Thank you for your email. I am sorry for the delay as lab took more time than anticipated.

    We may be able to provide you the positive control lysates for ab16088. This would be whole cell lysate of HEK 293T cells transfected with an expression...

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    Thank you for contacting us.

    I can confirm that, I have sent reminder to our collaborator lab. I will send you the requested details as soon as I receive a reply from them.

    Many thanks for having patience!

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"