概述

  • 产品名称Anti-APPL抗体
    参阅全部 APPL 一抗
  • 描述
    兔多克隆抗体to APPL
  • 经测试应用适用于: WB, IP, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Hamster, Human, Monkey
  • 免疫原

    Recombinant full length protein (Human) expressed in E.coli

  • 阳性对照
    • HEK293 cell lysate C2C12 cells

性能

应用

Our Abpromise guarantee covers the use of ab95195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 80 kDa).
IP Use at an assay dependent dilution.
ELISA Use a concentration of 0.5 - 1 µg/ml.
ICC/IF Use a concentration of 1 - 2 µg/ml.

靶标

  • 功能Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction.
  • 组织特异性High levels in heart, ovary, pancreas and skeletal muscle.
  • 序列相似性Contains 1 PH domain.
    Contains 1 PID domain.
  • 结构域Overexpression of an N-terminal domain (residues 1-319) or a C-terminal region (residues 273-709) has a proapoptotic effect.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Early endosome membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adapter protein containing PH domain antibody
    • Adaptor Protein Containing pH Domain antibody
    • Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody
    • Adaptor protein containing pH domain PTB domain and leucine zipper motif antibody
    • AKT2 INTERACTOR antibody
    • APPL 1 antibody
    • Appl1 antibody
    • DCC interacting protein 13 alpha antibody
    • DCC-interacting protein 13-alpha antibody
    • DIP 13 alpha antibody
    • DIP13 alpha antibody
    • Dip13-alpha antibody
    • DIP13alpha antibody
    • DP13A_HUMAN antibody
    • KIAA1428 antibody
    • PTB domain and leucine zipper motif 1 antibody
    • PTB Domain and Leucine Zipper Motif antibody
    • Signaling adaptor protein DIP13alpha antibody
    see all

Anti-APPL antibody 图像

  • All lanes : Anti-APPL antibody (ab95195) at 0.2 µg/ml

    Lane 1 : HEK293 cell lysate
    Lane 2 : C2C12 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat anti-rabbit antibody

    Predicted band size : 80 kDa
  • Immunostaining of APPL in C2C12 cells using ab95195 at 2 µg/ml followed by goat anti rabbit antibody, visualized by confocal microscopy.

Anti-APPL antibody (ab95195)参考文献

ab95195 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Hamster Cell lysate - whole cell (Pancreatic Beta Cells)
Loading amount 15 µg
Specification Pancreatic Beta Cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Apr 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"