Apoptotic DNA Ladder Detection试剂盒(ab66090)

概述

  • 产品名称Apoptotic DNA Ladder Detection试剂盒
    参阅全部 DNA fragments 试剂盒
  • 测试
    50 x 1 assay
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型Direct
  • 检测时间
    1h 30m
  • 产品概述

    Apoptotic DNA Ladder Detection Kit (ab66090) provides an easy and sensitive solution for detecting DNA fragmentation in apoptotic cells. It can isolate small fragmented DNA from cells in only 90 minutes, whereas other similar products in the market require 1-2 days. No DNA extraction or additional columns required.



    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.

应用

Our Abpromise guarantee covers the use of ab66090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

Apoptotic DNA Ladder Detection Kit 图像

  • Apoptosis was induced in 10e6 Jurkat cells by treatment with 10 μM Camptothecin (CPT) (ab120115) for 4 hours (2, 5) or 2 μM CPT for 24 hours (3, 6) whilst control cells were kept without treatment (1, 4). DNA was extracted and resuspended in 60 uL resuspension buffer. 10 uL (1-3) or 40 uL (4-6) were loaded onto the agarose gel.

实验方案

Apoptotic DNA Ladder Detection Kit (ab66090)参考文献

ab66090 has not yet been referenced specifically in any publications.

Product Wall

If you choose to do an overnight incubation with Enzyme B, we recommend doing so at 50 degrees Celsius. If you do this, make sure the tube is sealed well to prevent evaporation.



As long as you perform the incubation for at least 30 m...

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It is very easy to use.

Excellent Excellent 5/5 (Ease of Use)
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very good for menuscript data
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Miss. Jae Ok SEO

Verified customer

提交于 Jul 23 2014

While it is uncommon for the DNA pellet to not break up with vortexing and pippetting, should this occur warming the solutions should help. There is also a yellow dye included in the suspension buffer for visualizing on the gel.

The lab has confirmed that you may store the resuspended DNA at -20°C. DNA generally does not freeze at this temp. Please don’t store it at 4°C.

Thank you for your enquiry.

For India, you can order from one of our following official distributors. I can recommend to contact them and they can give you a price.

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Thank you for contacting Abcam.

I have had the chance to speak with the lab. Although they have not seen this type of issue before, they suggest when doing overnight incubation to ensure that the tubes are airtight to prevent evaporation of ...

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Thank you for your enquiry. I am pleased to confirm the following information for you regarding the components of this kit (ab66090) TE Lysis Buffer- Aids in cell lysis Enzyme A Solution- Removes RNA Enzyme B (Lyophilized)- Digests ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"