Apoptotic Cell Enrichment试剂盒(ab65782)
Key features and details
- Assay type: Direct
- Assay time: 1 hr
- Sample type: Adherent cells, Suspension cells
概述
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产品名称
Apoptotic Cell Enrichment试剂盒 -
样品类型
Adherent cells, Suspension cells -
检测类型
Direct -
检测时间
1h 00m -
产品概述
Abcam's Apoptotic Cell Isolation Kit provides a simple and efficient means for isolation of apoptotic cells or removal of dead cells from cell culture or tissue preparations using annexin V/magnetic beads (MagBeads). Annexin V is a Ca2+-dependent phospholipid binding protein with high affinity for phosphatidylserine (PS), which is redistributed from the inner to the outer plasma membrane leaflet in apoptotic or dead cells. Once on the cell surface, PS becomes available for binding to annexin V and any of its conjugates. Binding of annexin V-biotin to apoptotic cells followed by binding of the biotin to streptavidin-MagBeads enables separation of apoptotic cells from living cells. The apoptotic cells bound to the MagBeads adhere to the magnet, while non-apoptotic cells stay in suspension. The separated apoptotic and healthy cells can then be used in a variety of assays to study apoptotic mechanisms and pathways. The kit has also been successfully used to remove dead cells from healthy cell culture.
Visit our FAQs page for tips and troubleshooting. -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K258 Apoptotic Cell Isolation Kit. K258-30 is the same size as the 30 test size of ab65782.
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经测试应用
适用于: FM, Flow Cytmore details
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 30 tests 1x Binding Buffer 1 x 25ml Annexin V-Biotin 1 x 150µl Apoptotic Cell Releasing Buffer 1 x 10ml Streptavidin-MagBeads 1 x 1.5ml -
研究领域
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相关性
Cell isolation is a way of dissociating cells from tissues and separating the various types in order to obtain a relatively homogeneous population of cells for analysis.
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应用
The Abpromise guarantee
Abpromise™承诺保证使用ab65782于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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FM |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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说明 |
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FM
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab65782 尚未被引用在任何文献中。