Anti-Apoptosis inhibitor 5抗体(ab99307)

概述

  • 产品名称Anti-Apoptosis inhibitor 5抗体
    参阅全部 Apoptosis inhibitor 5 一抗
  • 描述
    兔多克隆抗体to Apoptosis inhibitor 5
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis , Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Immunogen corresponds to a region within amino acids 1-50 of Human Apoptosis inhibitor 5 (XP_508375.2).

  • 阳性对照
    • Whole cell lysates from HeLa, 293T and Mouse NIH 3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab99307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
  • 组织特异性Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer.
  • 序列相似性Belongs to the API5 family.
  • 结构域Two regions, an N-terminal (aa 96-107) and a C-terminal (aa 274-311) are required for binding FGF2.
  • 细胞定位Cytoplasm and Nucleus. Cytoplasm. Mainly nuclear. Can also be cytoplasmic.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AAC 11 antibody
    • AAC-11 antibody
    • AAC11 antibody
    • Antiapoptosis clone 11 protein antibody
    • API 5 antibody
    • API-5 antibody
    • api5 antibody
    • API5 like 1 antibody
    • API5_HUMAN antibody
    • API5L1 antibody
    • Apoptosis inhibitor 5 antibody
    • Cell migration inducing gene 8 protein antibody
    • Cell migration-inducing gene 8 protein antibody
    • Fibroblast growth factor 2 interacting factor 2 antibody
    • Fibroblast growth factor 2-interacting factor antibody
    • FIF antibody
    • MIG 8 antibody
    • MIG8 antibody
    • Migration inducing protein MIG8 antibody
    • Protein XAGL antibody
    see all

Anti-Apoptosis inhibitor 5 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling Apoptosis inhibitor 5 with ab99307 at 1/1000 (0.2µg/mg) and 1/200 (1 µg/mg). Detection: DAB.
  • All lanes : Anti-Apoptosis inhibitor 5 antibody (ab99307) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH 3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 58 kDa


    Exposure time : 10 seconds
  • ab99307, at 0.4 µg/ml, staining Apoptosis inhibitor 5 in HeLa whole cell lysate immunoprecipitated using ab99307 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with an exposure time of 1 second.

Anti-Apoptosis inhibitor 5 antibody (ab99307)参考文献

ab99307 has not yet been referenced specifically in any publications.

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