Anti-Apolipoprotein M抗体(ab76458)


  • 产品名称Anti-Apolipoprotein M抗体
    参阅全部 Apolipoprotein M 一抗
  • 描述
    兔多克隆抗体to Apolipoprotein M
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Pig, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Apolipoprotein M.

    (Peptide available as ab87119.)

  • 阳性对照
    • This antibody gave a positive signal in Human Plasma.



Our Abpromise guarantee covers the use of ab76458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 21 kDa).


  • 功能Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
  • 组织特异性Plasma protein. Expressed in liver and kidney.
  • 序列相似性Belongs to the calycin superfamily. Lipocalin family. Highly divergent.
  • 细胞定位Secreted. Present in high density lipoprotein (HDL) and to a lesser extent in triglyceride-rich lipoproteins (TGRLP) and low density lipoproteins.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apo M antibody
    • Apo-M antibody
    • Apolipoprotein M antibody
    • ApoM antibody
    • APOM_HUMAN antibody
    • G3A antibody
    • HSPC336 antibody
    • MGC22400 antibody
    • NG20 antibody
    • NG20 like protein antibody
    • Protein G3a antibody
    see all

Anti-Apolipoprotein M antibody 图像

  • Anti-Apolipoprotein M antibody (ab76458) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab76458 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76458, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HepG2 cells at 5µg/ml.
  • IHC image of Apolipoprotein M staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76458, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Apolipoprotein M antibody (ab76458)参考文献

ab76458 has not yet been referenced specifically in any publications.

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Merci de nous avoir contactés.

J’ai vérifié ab76458. Puisque ce produit a une concentration >1mg/ml de BSA, je peux vous confirmer qu’il ne contient pas de BSA. Notez bien que ceci n’est pas le cas p...

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Merci de nous avoir contactés.

ab91656 n’a pas été purifié. Il est vendu sous forme de surnageant et dans un tampon de 49% PBS, 0.01% Azoture de sodium, 50% Glycérol, 0.05% BSA.

ab76458 a &ea...

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Merci de nous avoir contactés.

Comme je vous l’ai expliqué au téléphone, la séquence immunogène d’ab91656 est xxxxxxxx qui correspond aux acides aminés xxxxxxxchez l’humain. J...

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