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Synthetic peptide spanning the polymorphic amino acid position 158 of ApoE.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab183597 as a replacement.
Our Abpromise guarantee covers the use of ab1907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 38 kDa.|
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 µg/ml.|
10% SDS -PAGE.
Blocked with 5% milk for 2 hours at 22°C.
Incubated with the primary antibody for 18 hours at 4°C in TBST + 5% milk.
IHC-FoFr image of Apolipoprotein E staining on choroid plexus of APP transgenic mouse brain sections using ab1907 (1:500). The mouse was perfused with 4% PFA in 0.1M PBS. The sections were permeabilised using 0.1% TriktonXin 0.1% PBS. 10% serum was used for 1 hour at 24°C for blocking step. The sections were stained using ab1907 at 1:500 dilution and Rabbit monoclonal to anti-mouse IgG conjugated to Alexa Fluor® 568 was used at 1:1000 to detect the primary antibody. DAPI was used to stain the nuclei.
ICC/IF image of ab1907 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1907, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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