RabMAb

Anti-Apolipoprotein D抗体[EPR2916] (ab108191)

概述

  • 产品名称Anti-Apolipoprotein D抗体[EPR2916]
    参阅全部 Apolipoprotein D 一抗
  • 描述
    兔单克隆抗体[EPR2916] to Apolipoprotein D
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Apolipoprotein D.

  • 阳性对照
    • WB: Human plasma cell lysate IHC-P: Human kidney or liver tissue
  • 常规说明

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab108191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

靶标

  • 功能APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts.
  • 组织特异性Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears.
  • 序列相似性Belongs to the calycin superfamily. Lipocalin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APO D antibody
    • Apo-D antibody
    • ApoD antibody
    • APOD protein antibody
    • APOD_HUMAN antibody
    • Apolipoprotein D antibody
    • ApolipoproteinD antibody
    see all

Anti-Apolipoprotein D antibody [EPR2916] 图像

  • Anti-Apolipoprotein D antibody [EPR2916] (ab108191) at 1/2000 dilution (purified) + human plasma at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size : 21 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded human liver with purified ab108191 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Anti-Apolipoprotein D antibody [EPR2916] (ab108191) at 1/1000 dilution (unpurified) + Human plasma cell lysate at 10 µg

    Predicted band size : 21 kDa
  • Immunohistochemical staining of Apolipoprotein D in paraffin embedded human kidney tissue, using unpurified ab108191 at a 1/100 dilution.

  • Immunohistochemical staining of Apolipoprotein D in paraffin embedded Human liver tissue, using unpurified ab108191 at a 1/100 dilution.

Anti-Apolipoprotein D antibody [EPR2916] (ab108191)参考文献

ab108191 has not yet been referenced specifically in any publications.

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