Anti-Apolipoprotein CIII抗体(Biotin) (ab21024)

概述

  • 产品名称Anti-Apolipoprotein CIII抗体(Biotin)
    参阅全部 Apolipoprotein CIII 一抗
  • 描述
    山羊多克隆抗体to Apolipoprotein CIII (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: Sandwich ELISA, WB, ELISAmore details
  • 种属反应性
    与反应: Human, Monkey
  • 免疫原

    Purified human Apolipoprotein CIII.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab21024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration. PubMed: 21371998
WB 1/8000 - 1/30000. Predicted molecular weight: 11 kDa. Dilution optimised using Chromogenic detection.
ELISA 1/8000 - 1/30000. Can be paired for ELISA with Rabbit polyclonal to Apolipoprotein CIII (ab21032).

靶标

  • 功能Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles.
  • 组织特异性Constitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine.
  • 疾病相关Defects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
  • 序列相似性Belongs to the apolipoprotein C3 family.
  • 翻译后修饰O-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APOC3 antibody
    • APO C3 antibody
    • Apo CIII antibody
    • Apo-CIII antibody
    • APOC 3 antibody
    • ApoC III antibody
    • ApoC-III antibody
    • APOC3 antibody
    • APOC3_HUMAN antibody
    • ApoCIII antibody
    • Apolipoprotein C III antibody
    • Apolipoprotein C-III antibody
    • Apolipoprotein C3 antibody
    • ApolipoproteinCIII antibody
    • MGC150353 antibody
    see all

Anti-Apolipoprotein CIII antibody (Biotin) 图像

  • Sandwich ELISA, detecting Apolipoprotein CIII levels in Human or cynomolgus monkey serum, using ab21024 as detection antibody.

    Anti-human Apolipoprotein CIII polyclonal antibody (ab21032) was added to an ELISA plate and incubated overnight at 4°C. Blocking buffer was added and incubated for 1 hour at room temperature. 50 µl of serum or standard was added to each well and incubated for 2 hours at room temperature. After washing, 50 µl/well of detection antibody (ab21024 at 1 µg/ml) was added and incubated at room temperature 1 hour followed by 50 µl/well of Streptavidin-HRP (ab64269). After color development, the absorbance was read at 450nm.

Anti-Apolipoprotein CIII antibody (Biotin) (ab21024)参考文献

This product has been referenced in:
  • Wang Y  et al. Development of a sensitive ELISA to quantify apolipoprotein CIII in nonhuman primate serum. J Lipid Res 52:1265-71 (2011). Sandwich ELISA ; Cynomolgus Monkey . Read more (PubMed: 21371998) »
  • Dernick G  et al. Multidimensional profiling of plasma lipoproteins by size exclusion chromatography followed by reverse-phase protein arrays. J Lipid Res 52:2323-31 (2011). Read more (PubMed: 21971713) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab21024.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"